Study on the Antifungal Constituents from Hoh Xil-Derived Streptomyces sp. 444

doi:10.6023/cjoc202604020

Chinese Journal of Organic Chemistry

ARTICLE

可可西里来源链霉菌444的抗真菌活性成分研究

安俊榴^a, 徐吉祥^b, 庞昆^a, 杨信怡^b, 孙承航^b,c,*, 袁丽杰^a,*

^a华北理工大学基础医学院河北省慢性疾病基础医学重点实验室唐山市慢性病临床基础研究重点实验室唐山 063000;
^b中国医学科学院&北京协和医学院医药生物技术研究所北京 100050;
^c青海大学生态环境工程学院西宁 810016

收稿日期:2026-06-28 修回日期:2026-08-05 发布日期:2026-09-14
基金资助:
国家重点研发计划(No. 2024YFC2309300)和河北省自然科学基金项目 (No. H2021209027)及青海省“昆仑英才高端创新创业人才”计划.

Study on the Antifungal Constituents from Hoh Xil-Derived Streptomyces sp. 444

An Junliu^a, Xu Jixiang^b, Pang Kun^a, Yang Xinyi^b, Sun Chenghang^b,c,*, Yuan Lijie^a,*

^aTangshan Key Laboratory for Preclinical and Basic Research on Chronic Disease, Hebei Key Laboratory for Chronic Diseases, School of Basic Medical Science, North China University of Science and Technology, Tangshan 063000;
^bInstitute of Medicinal Biotechnology, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050;
^cCollege of Eco-Environmental Engineering, Qinghai University, Xining 810016

Received:2026-06-28 Revised:2026-08-05 Published:2026-09-14
Contact: *E-mail: yuanlijie1970@163.com; sunchenghang@imb.pumc.edu.cn
Supported by:
National Key Research and Development Program of China (No. 2024YFC2309300) and the Natural Science Foundation of Hebei Province (No. H2021209027). The Kunlun Talented People, High-end Innovation and Entrepreneurship Talents Plan of Qinghai Province.

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Abstract

Activity-guided and DeepSAT analyses on the antifungal constituents of Streptomyces sp. 444, isolated from the Hoh Xil region, led to the discovery of two new antimycin analogs, deformyl-antimycin A_10a (1) and antimycin A_1c (2), along with two known analogs, antimycin A_3a (3) and antimycin A_8a (4). The structures of these antimycin analogs were elucidated by high-resolution electrospray ionization mass spectroscopy (HR-ESI-MS), nuclear magnetic resonance (NMR), and NMR calculations. Bioassay indicated that compounds 1-4 exhibited antifungal activity. The MIC values of compound 2 against Candida albicans ATCC 10231 and Cryptococcus neoformans H99, determined by the broth microdilution method, were 4 and 8 μg/mL, respectively.

Key words: Hoh Xil, Streptomyces sp., DeepSAT, antimycin, antifungal activity

Cite this article

An Junliu, Xu Jixiang, Pang Kun, Yang Xinyi, Sun Chenghang, Yuan Lijie. Study on the Antifungal Constituents from Hoh Xil-Derived Streptomyces sp. 444[J]. Chinese Journal of Organic Chemistry, doi: 10.6023/cjoc202604020.

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[1] Newman D. J.; Cragg G. M.J. Nat. Prod. 2020, 83(3): 770-803.
[2] Butler M. S.; Capon R. J.; Blaskovich M. A.T.; Henderson, I. R.Nat. Prod. Rep. 2025.
[3] Yang J.; Wang J.; Liu M.; Zhou X.; Feng D.; Jiang H.; Liu X.; Chen L.; Wang Y.Chin. J. Nat. Med. 2025, 23, 1047.
[4] Genilloud O.Nat. Prod. Rep. 2017, 34, 1203.
[5] Carolus H.; Pierson S.; Lagrou K.; Van Dijck, P.J. Fungi. 2020, 6.
[6] Howard K. C.; Dennis E. K.; Watt D. S.; Garneau-Tsodikova, S.Chem. Soc. Rev. 2020, 49, 2426.
[7] Huo R.; Shi D.; Zi G.; Cai L.; Liu L.J. Agric. Food. Chem. 2025, 73, 26793.
[8] Kim H. W.; Zhang C.; Reher R.; Wang M.; Alexander K. L.; Nothias L. F.; Han Y. K.; Shin H.; Lee K. Y.; Lee K. H.; Kim M. J.; Dorrestein P. C.; Gerwick W. H.; Cottrell G. W. J. Cheminf. 2023, 15, 71.
[9] Park W. H.Chem. Biol. Interact. 2025, 422, 111805.
[10] Wang F.; Fu S. N.; Bao Y. X.; Yang Y.; Shen H. F.; Lin B. R.; Zhou G. X.Nat. Prod. Res. 2017, 31, 1819.
[11] Li W.; Ding L.; Li J.; Wen H.; Liu Y.; Tan S.; Yan X.; Shi Y.; Lin W.; Lin H. W.; He S.J. Agric. Food. Chem. 2022, 70, 8309.
[12] Hosotani N.; Kumagai K.; Nakagawa H.; Shimatani T.; Saji I.J. Antibiot. 2005, 58, 460.
[13] Jensen, F. J. Chem. Theory. Comput. 2006, 2, 1360.
[14] Kanehara R.; Shirakawa K.; Sakashita H.; Oinuma Y.; Ohta S.; Okazaki M.; Tanaka K.; Tonouchi A.; Hashimoto M. J. Nat. Prod. 2026, 89, 584.
[15] Barrow C. J.; Oleynek J. J.; Marinelli V.; Sun H. H.; Kaplita P.; Sedlock D. M.; Gillum A. M.; Chadwick C. C.; Cooper R.J. Antibiot. 1997, 50, 729.
[16] Tsai Y.-H.; Amichetti M.; Zanardi M. M.; Grimson R.; Daranas A. H.; Sarotti A. M.Org. Lett. 2022, 24, 7487.
[17] Mbeng Obame, R. B.; Gallard, S.; Gibert, S.; Gallard, J.-F.; Ferron, S.; Séon-Méniel, B.; Bernadat, G.; Beniddir, M. A.; Le Pogam, P.J. Nat. Prod. 2025, 88, 935.
[18] Wang F.; Chen F.; Zheng X.-H.; Lin B.-R.; Zhou G.-X.Chin. J. Antibiot. 2017, 42(11): 932-939 (in chinese)
(王帆, 陈芳, 郑新恒, 林壁润, 周光雄, 中国抗生素杂志, 2017, 42(11): 932-939.)
[19] Xie Y.; Wang W.-J.; Shen K.-L.Chin J Appl Clin Pediatr. 2023, 38(4): 266-270 (in Chinese)
(谢淦, 王文建, 申昆玲, 中华实用儿科临床杂志, 2023, 38(4): 266-270.)
[20] Wang X.-Y.; Liu R.-S.; Zhong H.Cent South Pharm. 2023, 21(6): 1403-1411 (in Chinese)
(王曦悦, 刘润松, 仲华, 王彦, 中南药学, 2023, 21(6): 1403-1411.)
[21] Chemical Computing Group. Molecular Operating Environment (MOE), ver.2019.01; Montreal, Quebec, Canada.
[22] Halgren T.A.J. Comput. Chem. 1996, 17: 490-519
[23] Jin T.-Y., Li P.-L., Wang C.-L., Tang X.-L., Cheng M.-M., Zong Y., Luo L.-Z., Ou H.-L., Liu K.-C. and Li G.-Q.Chin. J. Chem. 2021, 39: 2588-2598
[24] Tian L, Molclus program, Version 1.13, http://www.keinsci.com/research/molclus.html
[25] Lu T.; Chen Q.Comput. Theor. Chem. 2021, 1200, 113249.
[26] Jensen F.J. Chem. Theory. Comput. 2006, 2, 1360
[27] Barbara D.A.; Gary W. P.;Philippe D.; Jeff F.; Mahmoud A. G.; Kimberly E. H.; Denise H.; Nicole M. H. Laura K.; Shawn R. L.; Luis O.; Audrey N. S.; Nathan P. W.; Adrian M. Z.; CLSI supplement M27. Clinical and Laboratory Standards In-stitute (CLSI). Wayne, PA; 2017, pp. 7~17.
[28] Weber M.; Dékány M.; Nagyné A. N.; Felegyi-Tóth, C. A.; Suratno, S.; Krámos, B.; Bodó, E.; Béni, Z.; Ványolós, A. J. Nat. Prod. 2025, 88, 644.

可可西里来源链霉菌444的抗真菌活性成分研究

Study on the Antifungal Constituents from Hoh Xil-Derived Streptomyces sp. 444

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