Application of mass spectrometry in protein and peptide chemistry

Abstract

This article introduces four newly developed mass spectrometric methods. There are fast atom bombardment-MS, (FAB-MS), tandem mass spectrometry (MS/MS), electrospray ionization-MS(ESI-MS) and matrix assisted laser desorption ionization time of flight-MS(MALDI-TOF-MS). These developments make MS be used for the structural analysis of protein. This paper also discusses their application in the following aspects by several examples: the molecular weight determination of proteins, appraise of the purity of proteins or peptides, the structural determination of a glycopeptide and the structural determination of the N-blocked or normal peptides and proteins.

Key words: FAB MASS SPECTROMETRY, MASS SPECTROGRAPHY, AMINO ACID SEQUENCE ANALYSIS, RICHOSATHIN PROTEIN, GLYCOPEPTIDE, POLYPEPTIDE, PROTEIN, STRUCTURAL ANALYSIS, MOLECULAR WEIGHT

CLC Number:

Q51
O657

Cite this article

LI YILI;JIN SHANWEI. Application of mass spectrometry in protein and peptide chemistry[J]. Chin. J. Org. Chem., 1996, 16(3): 209-217.

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