Analysis of ribonucleosides and free bases by HPLC with a diode array spectrophotometer

Abstract

Qual. and quant. determination of ribonucleosides and their free bases by ion-exchange HPLC with a diode array spectrophotometer was performed. The components uracil, cytosine, adenine, uridine, guanosine, cytidine, adenosine in the chem. hydrolyzate of yeast RNA were separated on a Nucleosil-scx-5 m column by using citric acid-phosphate buffer, pH 5 as mobile phase. The identification of UV spectrum of the slope up, slope down, and apex portions of each peak at 240-350 nm confirms that not only each peak contains a single component, but also what component it is.

Key words: CYTIDINE, HIGH SPEED LIQUID CHROMATOGRAPHY, GUANOSINE, URIDINE, ADENOSINE, SPECTROPHOTOMETERS, CYTOSINE, ADENINE, URACIL, RIBONUCLEOSIDE, DIODE DETECTOR

CLC Number:

O656

Cite this article

LI WENHUA;WANG QIUXUAN;SHEN QI;WANG JINHUA. Analysis of ribonucleosides and free bases by HPLC with a diode array spectrophotometer[J]. Chin. J. Org. Chem., 1988, 8(2): 149-152.

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