Based on the 5-(4-hydroxybenzyl)-2,4-imidazolidinedione esters synthesized by simulating the structure of natural inhibitor of AdSS synthetase, twenty eight novel compounds 5-(4-hydroxyphenyl)-2,4- imidazolidinedione esters were synthesized using 5-(4-hydroxyphenyl)-2,4-imidazolidinedione as the key intermediate. Their structures were confirmed by m.p., IR, ¹H NMR techniques and elemental analysis. The preliminary bioassay results indicated that 5-(4-hydroxyphenyl)-2,4-imidazolidinedione p-tert-butylbenzoate (2i) and 2-thiophenate (3b) have an inhibitory ratio 70% against Arabidopsis thaliana at the concentration of 200 μg/mL.

Key words: AdSS inhibitor, 5-(4-hydroxyphenyl)-2,4-imidazolidinedione ester, bioactivity