化学学报 ›› 2022, Vol. 80 ›› Issue (4): 553-562.DOI: 10.6023/A21120578 上一篇    下一篇

综述

基于抑制扭转的分子内电荷转移(TICT)提升有机小分子荧光染料亮度及光稳定性

许宁a,b, 乔庆龙a,*(), 刘晓刚c, 徐兆超a,b,*()   

  1. a 中国科学院大连化学物理研究所 辽宁 大连 116023
    b 大连理工大学张大煜学院 辽宁 大连 116024
    c 新加坡科技设计大学 新加坡 487372
  • 投稿日期:2021-12-23 发布日期:2022-04-28
  • 通讯作者: 乔庆龙, 徐兆超
  • 作者简介:

    许宁, 博士研究生, 2018年7月毕业于山东理工大学化学化工学院, 获理学硕士学位. 目前就读于大连理工大学张大煜(化学)学院, 主要从事新型荧光团的开发以及荧光成像工作.

    乔庆龙, 大连化学物理研究所副研究员. 2017年获得大连理工大学博士学位, 2018年加入大连化学物理研究所徐兆超教授课题组, 2020年任副研究员, 主要研究方向为新型荧光染料开发、超分辨动态成像和蛋白质荧光探针.

    徐兆超, 大连化学物理研究所研究员, 博士生导师. 2006年毕业于大连理工大学, 师从钱旭红教授. 随后, 进入梨花女子大学的Juyoung Yoon教授课题组, 担任博士后研究员. 2008年10月起在剑桥大学David R. Spring教授课题组担任Herchel Smith Research Fellow. 2011年至今在中国科学院大连化学物理研究所任课题组组长. 主要研究方向为新型的荧光团设计和发光构效关系研究、蛋白质标记和传感、荧光探针、超分辨率荧光成像.

    庆祝中国科学院青年创新促进会十年华诞.
  • 基金资助:
    国家自然科学基金(22078314); 国家自然科学基金(21878286); 国家自然科学基金(21908216); 大连化学物理研究所(DICPI202142); 大连化学物理研究所(DICPI201938); 大连化学物理研究所(DICPZZBS201805); 新加坡教育部(MOE-MOET2EP10120-0007); 新加坡科技设计大学(SUTD-ZJU(SUTD-ZJU (VP) 201905)

Enhancing Brightness and Photostability of Organic Small Molecular Fluorescent Dyes Through Inhibiting Twisted Intramolecular Charge Transfer (TICT)

Ning Xua,b, Qinglong Qiaoa(), Xiaogang Liuc, Zhaochao Xua,b()   

  1. a Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, Liaoning Province, China
    b Zhang Dayu School of Chemistry, Dalian University of Technology, Dalian 116024, China
    c Singapore University of Technology and Design, Singapore 487372, Singapore
  • Received:2021-12-23 Published:2022-04-28
  • Contact: Qinglong Qiao, Zhaochao Xu
  • About author:
    Dedicated to the 10th anniversary of the Youth Innovation Promotion Association, CAS.
  • Supported by:
    National Natural Science Foundation of China(22078314); National Natural Science Foundation of China(21878286); National Natural Science Foundation of China(21908216); Dalian Institute of Chemical Physics(DICPI202142); Dalian Institute of Chemical Physics(DICPI201938); Dalian Institute of Chemical Physics(DICPZZBS201805); The authors thank the support from the Ministry of Education, Singapore(MOE-MOET2EP10120-0007); Singapore University of Technology and Design(SUTD-ZJU (VP) 201905)

有机小分子荧光染料研究已有170余年历史, 其结构和性能随着合成方法和应用需求的发展而不断革新, 已被广泛应用于荧光标记、探针和生物成像中. 近年来发展起来的超分辨荧光成像技术对有机小分子荧光染料的亮度、稳定性和开关性能等均提出了更高的要求, 这为染料发展带来了新的机遇. 当前, 化学工作者也将更多精力聚焦在染料结构改造提升有机小分子荧光染料的亮度与光稳定性. 激发态扭转的分子内电荷转移(TICT)是有机小分子荧光染料中主要的非辐射衰减途径之一. 因而, 抑制TICT能够很好地提升染料的亮度和光稳定性, 并成为目前针对超分辨成像技术发展高亮度和光稳定性的有机小分子荧光染料的主要方法. 本综述首先简要回顾了TICT的机制和发展过程, 而后重点介绍近些年通过抑制TICT策略来提升不同结构有机小分子荧光染料光谱性能方面的进展.

关键词: 超分辨成像, 有机小分子荧光染料, 扭曲的分子内电荷转移, 亮度, 光稳定性

During the past 170 years, organic small molecular fluorescent dyes had been widely applied in fluorescence labeling, fluorescence probes and bioimaging. And their structures and performances continually evolved with development of synthetic method and application. However, the emerging super-resolution imaging put forward higher requirements at brightness, stability and switching performance of organic small molecular fluorescent dyes, which also offers new opportunity for developing novel dyes at the same time. So, chemists presently pay more attentions on brightness and photostability. Twisted intramolecular charge transfer (TICT), the major nonradiative decay channel in organic small molecular fluorescent dyes, seriously decrease brightness and photostability. Therefore, inhibiting TICT has became the crucial strategy to develop organic small molecular fluorescent dyes towards super-resolution imaging. This review will firstly demonstrate mechanism and development of TICT and emphatically introduce the progress in improving organic small molecular fluorescent dyes based on inhibiting TICT.

Key words: super-resolution imaging, organic small molecular fluorescent dyes, twisted intramolecular charge transfer, brightness, photostability