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化学学报
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化学学报 ›› 1998, Vol. 56 ›› Issue (8): 827-832. 上一篇    

研究论文

茜素红 S与蛋白质的反应机理研究

庄稼;迟燕华;李克安;李娜;童沈阳   

  1. 北京大学化学与分子工程学院;西南工学院材料科学与工程系
  • 发布日期:1998-08-15

Study on the reaction between alizarin red S and protein

ZHUANG JIA;CHI YANHUA;LI KEAN;LI NA;TONG SHENYANG   

  • Published:1998-08-15

PDF

534

被引次数

121 | 25

应用UV光谱法研究在pH4.35的缓冲溶液中, 茜素红 S(ARS)与牛血清蛋白(BSA)间的相互结合反应。测定了平均结合数n=8, 分配结合常数K~c=1.5×10^5。研究了小分子探针与蛋白质的反应机理, 相互间的结合部位及结合力。该复合物结合前后的等吸收波长红移28nm, 这是目前此类结合反应研究中, 其分子间结合力较大的一例。进一步研究了ARS与多种蛋白质的结合反应, 以及离子强度对该体系的影响。提出了合适的反应模型。

关键词: 紫外分光光度法, 反应机理, 茜素红, 蛋白质, 血清白蛋白, 结合反应

The interaction of alizarin red S and bovine serum albumin was studied by UV spectra in pH 4.35 buffer solution. The binding number (n=8) and binding constant (K~c=1.5×10^5) of the probe and protein were obtained. The main sort of binding force was investigated. It was found that the red shift of isosbestic point of the complex was 28nm and the sodium chloride concentration had significant effect on the binding reaction. The "Phase Distribution Model" is the proper description of the interaction between alizarin S and the protein.

Key words: ULTRAVIOLET SPECTROPHOTOMETRY, REACTION MECHANISM, ALIZARINE RED, PROTEIN, SERUM ALBUMIN

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