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化学学报
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化学学报 ›› 2003, Vol. 61 ›› Issue (1): 34-39. 上一篇    下一篇

研究论文

血红蛋白的过氧化物酶催化特性研究

王全林;刘志洪;蔡汝秀;吕功煊   

  1. 中国科学院兰州化学物理研究所,兰州(720000);武汉大学化学与环境科学学 院,武汉(430072)
  • 发布日期:2003-01-15

Study on Peroxidative Characteristics of Hemoglobin

Wang QuanlinR;Liu Zhihong;Cai Ruxiu;Lu Gongxuan   

  1. State Key Laboratory for Oxo Synthesis and Selective Oxidation, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou(730000);School of Chemistry and Environment, Wuhan University, Wuhan(430072);State Key Laboratory for Oxo Synthesis and Selective OxidationLanzhou Institute of Chemical Physics, Chinese Academy of Sciences,Lanzhou(730000)
  • Published:2003-01-15

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被引次数

144 | 29

探讨了用血红蛋白（Hb)作为辣根过氧化物酶（HRP）的替代物，用于率H2O2氧 化对甲基酚的反应体系，研究了血红蛋白的过氧化物酶特征及其酶催化动力学特性 。稳态速率法测定了米氏常数（Km)、米氏速率（Vm)及反应级数等动力学参数。讨 论了对甲基酚作为血红蛋白的氢供体底物的酶催化反应机理，确立了反应速率方程 。实验比较发现血红蛋白作为过氧化物酶（HRP）的替代物，其催化活性比氯化血 红素（Hemin)，β-环糊精－氯化血红素（β－CD－Hemin)等过氧化物模拟酶要高。

关键词: 过氧化物酶, 血红蛋白, 酶, 特性, 荧光, 过氧化氢, 甲基, 酚 P, 催化, 动力学

Hemoglobin was used as a substitute of peroxidase in the catalytic oxidation of P-cresol by H_2O_2. The peroxidative characteristics of hemoglobin and enzymatic kinetics were studied. The K_m value (1.85× 10~(-3)mol·L_(-1)) and V_m value (1.6 min_(-1)) were measured by steady-state catalytic velocity at pH=10.3 and temperature 25℃. The reaction mechanism was discussed when p-cresol was used as a hydrogen donor for hemoglobin. The velocity equation has been obtained. The results indicate that the catalytic activity of hemoglobin is higher tan those of other mimic enzymes (e.g. Hemin, β-CD-Hemin).

Key words: PEROXIDASE, HEMOGLOBIN, ENZYME, CHARACTERISTICS, FLUORESCENCE, HYDROGEN PEROXIDE, METHYL GROUP, PHENAL P, CATALYSIS, DYNAMICS

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