化学学报 ›› 2011, Vol. 69 ›› Issue (04): 445-451. 上一篇    下一篇

研究论文

中位醛基取代Cy5的光谱性能及作为粘度荧光探针的研究

杨志刚1,和艳霞1戴彬1窦柏蕊2王静云2彭孝军*,1   

  1. (1大连理工大学精细化工国家重点实验室 大连 116024)
    (2大连理工大学生命科学与技术学院 大连 116024)
  • 收稿日期:2010-07-14 修回日期:2010-08-26 出版日期:2011-02-28 发布日期:2010-10-21
  • 通讯作者: 彭孝军 E-mail:pengxj@dlut.edu.cn
  • 基金资助:

    国家自然科学基金项目;973计划项目;辽宁省教育厅创新团队项目

The Spectral Properties of Cy5 with Aldehyde Group Substituted at Meso-position of the Polymethine Chain Utilized as Fluorescent Viscosity Sensor

Yang Zhigang1 He Yanxia1 Dai Bin1 Dou Bairui2 Wang Jingyun2 Peng Xiaojun*,1   

  1. (1 State Key Laboratory of Fine Chemicals, Dalian University of Technology, Dalian 116024)
    (2 School of Life Science and Biotechnology, Dalian University of Technology, Dalian 116024)
  • Received:2010-07-14 Revised:2010-08-26 Online:2011-02-28 Published:2010-10-21

中位醛基取代的五甲川菁染料(Cy5)与传统Cy5相比其最大吸收和发射波长稍微蓝移, 斯托克斯位移增加, 在水中能达到48 nm|具有低的荧光量子产率, 而且随溶剂极性变化较小. 实验发现这种染料对环境的粘度非常敏感, 随着染料分子所处环境的粘度逐渐增加, 其荧光强度明显增加, 而且荧光强度与溶液粘度的关系满足F?rster-Hoffmann关系式. 该染料具有活细胞膜的穿透性, 能跨膜进入活细胞内, 使得该染料在检测活细胞内微环境粘度变化方面具有潜在的应用价值.

关键词: 菁染料, Cy5, 粘度, 荧光探针, 细胞成像

Compared with the traditional cyanine dyes (Cy5), both the maximum absorption and emission wavelengths of new Cy5 dyes with aldehyde group substituted at central position of the conjugated chain slightly blue shift, and the Stokes Shift is enlarged evidently to more than 28 nm, and largest up to 48 nm in water. The fluorescence quantum yield is rather low without obvious change in different solvents. It is found that the fluorescence of dye is sensitive to viscosity of the solvents, and the emission intensity of the dye can be enhanced largely by ascending of environmental viscosity with well linear relationship between lg If and lg (viscosity) (F?rster-Hoffmann equation). It is living cell membrane permeable for the dye 4 to map cellular microenvironmental viscosity, which demonstrates the potential application for dye 4 to investigate the intracellular microviscosity change.

Key words: cyanine dyes, Cy5, viscosity, fluorescent sensor, cell imaging