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化学学报
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化学学报 ›› 2000, Vol. 58 ›› Issue (1): 82-85. 上一篇    下一篇

研究论文

3-[二(羧甲基)氨甲基]-1, 2-二羟基蒽醌与蛋白质作用的研究

迟燕华;庄稼;董发勤;耿伟;李克安;童沈阳   

  1. 西南工学院材料科学与工程系;北京大学化学与分子工程学院
  • 发布日期:2000-01-15

The study on the reaction between 3-[di(carboxymethyl)aminomethyl]-1, 2-dihydroxyanthraquinone and bovine serum albumin

Chi Yanhua;Zhuang jia;Dong Faqin;Geng Wei;Li Kean;Tong Shenyang   

  • Published:2000-01-15

PDF

392

被引次数

66

在pH4.1的缓冲溶液中,研究3-[二(羧甲基)氨甲基]-1,2-二羟基蒽醌(AFB)与牛血清蛋白(BSA)结合反应,相互生成的紫色的配合物。采用UV光谱法,测得此配合物的最大吸收峰值为510nm,与试剂本身相比较红移90nm。表观摩尔吸光系数ε=1.2474×10^4mol^-^1.cm^-^1.L,最大结合数n=7,表观结合常数K~c=3.85×10^6。研究发现,AFB与BSA之间主要是以分子之间的静电引力结合,认为该反应符合Scatchard模型。离子强度对结合反应有显著的影响。

关键词: 蒽醌P, 蛋白质, 血清白蛋白, 紫外分光光度法, 相互作用

The interaction of 3-[di(carboxymethyl)aminomethyl]-1, 2- dihydroxyanthraquinone (AFB) and bovine serum albumin (BSA) was investigated by spectrophotometric method in acidic solution (pH4.1). When BSA was added into AFB solution, a purple color was observed, which indicated formation the of the AFB-BSA complex. The maximum absorption of the complex was obtained at 510nm, with the apparent molar absorptivity of ε=1.2474×10^4mol^-^1.cm^-^1.L. Two conditional constants were defined: the maximum binding number n=7 and apparent binding constant K~c=3.85×10^6. It is suggested that the electrostatic force is the main binding force. The Scatchard model is appropriate in the treatment of data obtained here. It is found that sodium chloride concentration of the solution has significant effect on the sensitivity of AFB-BSA complex.

Key words: ANTHRAQUINONE P, PROTEIN, SERUM ALBUMIN, ULTRAVIOLET SPECTROPHOTOMETRY, INTERACTIONS

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