建立了一种分子印迹-化学发光阵列传感器测定甘氨酸的新方法. 该方法以甘氨酸为模板分子, 合成了分子印迹聚合物微球, 将该聚合物微球固定在96孔板上, 用它来识别丹磺酰氯标记的甘氨酸(Dns-Gly). 最后加入化学发光试剂(TCPO-H2O2-咪唑), 测量相对化学发光强度定量检测甘氨酸. 在最佳试验条件下, 相对化学发光强度和甘氨酸的浓度在0.2～60 μmol/L范围内成良好的线性关系, 相关系数为r＝0.9972, 方法的检出限为0.07 μmol/L, 对1 μmol/L甘氨酸溶液进行11次平行测定, 相对标准偏差为3.3% (n＝11). 由于以甘氨酸为模板分子合成出来的分子印迹聚合物空腔比较小, 避免了非特异性吸附, 使它在识别丹磺酰氯标记的甘氨酸时特异性、响应速度和灵敏度都有所增强.

A new molecularly imprinted polymer (MIP)-chemiluminescence (CL) detection approach towards recognition of dansyl-glycine (Gly) is presented. The polymer microspheres were synthesized using precipitation polymerization with glycine as template and immobilized in microtiter plates (96 wells) using poly(vinyl alcohol) (PVA) as glue. The analyte (dansyl-glycine) was selectively adsorbed on the MIP microspheres. After washing, the bound fraction was quantified based on a dansyl-glycine-bis- (2,4,6-trichlorophenyl) oxalate (TCPO)-H2O2 chemiluminescence reaction enhanced by imidazole. The chemiluminescence intensity has a linear relationship with the concentration of glycine in the range of 0.2～60 μmol/L, with the limit of detection (LOD) being 0.07 μmol/L (3σ). The relative standard deviation (RSD) for 11 parallel measurements of glycine (1 μmol/L) was 3.3%. The cavity of MIP synthesized with glycine as template is smaller, which can avoid non-specific adsorption and lead to enhancement of specificity, response speed and sensitivity when recognizing dansyl-glycine.

国家自然科学基金