SIOC English
化学学报
高级检索

化学学报 ›› 2010, Vol. 68 ›› Issue (01): 83-88. 上一篇    下一篇

研究论文

鲱鱼精DNA修饰纳米金催化Fehling反应——共振散射光谱法检测痕量Hg2＋

温桂清1，李建福2，梁爱惠1，蒋治良*,1，何星存1   

  1. (1广西师范大学环境与资源学院 广西环境工程与保护评价重点实验室 桂林 541004)
    （2桂林工学院材料与化学工程系 桂林 541001）
  • 投稿日期:2009-02-23 修回日期:2009-08-29 发布日期:2010-01-20
  • 通讯作者: 蒋治良 E-mail:zljiang@mailbox.gxnu.edu.cn

Resonance Scattering Spectral Detection of Trace Hg2＋ Using Herring Sperm DNA Modified Nanogold as Catalyst of the Fehling Reaction

Wen Guiqing1 Li Jianfu2 Liang Aihui1 Jiang Zhiliang*,1 He Xingcun1   

  1. (1Guangxi Key Laboratory of Environmental Engineering, Protection and Assessment, Guangxi Normal University, Guilin 541004)
    (2 Guilin University of Technology, Guilin 541004)
  • Received:2009-02-23 Revised:2009-08-29 Published:2010-01-20

PDF

1430

用鲱鱼精DNA (hsDNA)修饰10 nm的纳米金制备了Hg2＋的hsDNA修饰纳米金共振散射光谱探针(AuhsDNA). 在pH 7.0 Tris-HCl缓冲溶液中及0.017 mol/L NaCl存在下, Hg2＋与AuhsDNA形成稳定的Hg2＋-DNA结合物, 引起AuhsDNA中的纳米金析出并聚集形成纳米金簇. 该溶液用150 nm滤膜过滤后, 滤液中过量的AuhsDNA可催化Fehling试剂-葡萄糖反应生成氧化亚铜微粒, 该微粒在580 nm处有一个较强的共振散射峰. 随着汞离子浓度增大, 形成的纳米金簇越多, 滤液中AuhsDNA越少, 生成的氧化亚铜微粒减少, 580 nm处氧化亚铜微粒的共振散射光强度线性降低, 其共振散射光强度降低值?I580 nm与汞离子浓度在1～833 nmol/L范围内成线性, 回归方程、相关系数、检出限分别为 ?I580 nm＋0.9, 0.9990, 0.3 nmol/L Hg2＋. 该法用于废水中Hg2＋的检测.

关键词: 汞离子, hsDNA修饰纳米金探针, 纳米催化, 共振散射光谱法

Herring sperm DNA (hsDNA) was used to modify 10 nm nanogold to obtain a resonance scattering (RS) spectral probe (AuhsDNA) for Hg2＋. In the medium of pH 7.0 Tris-HCl-0.017 mol/L NaCl, Hg2＋ interacted with AuhsDNA to form stable Hg2＋-hsDNA complex and larger nanogold clusters, which could be removed by membrane filtration. The excess AuhsDNA in the filtration solution exhibited catalytic effect on the Fehling reagent-glucose reaction to form Cu2O particles that exhibit the strongest RS peak at 580 nm. With addition of Hg2＋, the excess AuhsDNA in the filtration solution decreased accordingly, and the RS intensity at 580 nm decreased. The decreased RS intensity (ΔI580 nm) was linear to Hg2＋concentration in the range of 1～833 nmol/L, with regression equation of ΔI580 nm＝＋0.9, coefficient of 0.9990 and detection limit of 0.3 nmol/L. The proposed method was applied to detect Hg2＋ in water samples with satisfactory results.

Key words: Hg2＋, herring sperm DNA modified nanogold probe, nanocatalysis, resonance scattering