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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (14): 1449-1453. 上一篇    下一篇

研究论文

圆二色光谱法研究酸度、槲皮素、锌离子对β-酪蛋白二级结构的影响

吕鉴泉*,1，王雪梅1，吕桂英2，孙婷荃1，唐金燕1，徐明波1   

  1. (1湖北师范学院 污染物分析与资源化技术湖北省重点实验室 黄石 435002)
    (2黄石理工学院化学与材料工程学院 黄石 435003)
  • 投稿日期:2009-08-05 修回日期:2010-01-30 发布日期:2010-03-29
  • 通讯作者: 吕鉴泉 E-mail:jianquanlu@eyou.com
  • 基金资助:

    教育部重点项目(206096);湖北省教育厅创新团队项目和重点项目(D200922005)

Effect of pH, Quercetin and Zinc Ion on the Structure of β-Casein by Circular Dichroism Method

Lü Jianquan*,1 Wang Xuemei1 Lü Guiying2 Sun Tingquan1 Tang Jinyan1 Xu Mingbo1   

  1. (1 Hubei Key Laboratory of Pollutant Analysis & Reuse Technology, Hubei Normal University, Huangshi 435002)
    (2 School of Chemical and Material Engineering, Huangshi Institute of Technology, Huangshi 435003)
  • Received:2009-08-05 Revised:2010-01-30 Published:2010-03-29
  • Contact: LU JIAN-QUAN E-mail:jianquanlu@eyou.com

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采用圆二色光谱(CD)技术研究了酸度、槲皮素(Qct)和锌离子对溶液中β-酪蛋白(β-CN)二级结构的影响. 结果表明, 酸度、Qct和锌离子均能够诱导β-CN二级结构发生改变. 在pH 7.6的条件下, β-CN各种结构分别为32.6% α-螺旋, 53.5% β-折叠, 13.9% (-转角和无规卷曲; Qct使β-CN的α-螺旋含量增加、β-折叠含量减少; 锌离子和Zn-Qct配合物导致β-CN的α-螺旋含量大幅度降低, β-折叠含量略有增加, 同时转角和无规的含量也大幅度增加.

关键词: 二级结构, β-酪蛋白, 圆二色光谱, 槲皮素, 锌离子

The effect of pH, quercetin (Qct) and Zn(II) on the structure of β-casein (β-CN) in solution were investigated using circular dichroism spectroscopy. The results indicated that the secondary structures of β-CN in the solution were induced by the pH, quercetin and Zn(II)-Qct complexes. In pH 7.6 PBS buffer, β-CN contain 32.6% α-helix, 53.5% β-sheet and 13.9% γ-turn and random coil, the addition of Qct cause increase the content of α-helix and decrease the content of β-sheet, while Zn(II) and Zn(II)-Qct complexes lead to formation of γ-turn and random coil with the decease of α-helix.

Key words: secondary structure, β-casein, circular dichroism, quercetin, Zn(II)

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