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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (02): 243-246. 上一篇    下一篇

研究简报

微悬臂生物传感器检测生物素-亲和素相互作用

张慧勇1,2，潘宏青1,2，唐纪琳*,1，张柏林*,1   

  1. (1中国科学院长春应用化学研究所电分析化学国家重点实验室 长春 130022)
    (2中国科学院研究生院 北京 100049)
  • 投稿日期:2010-03-09 修回日期:2010-06-29 发布日期:2010-08-16
  • 通讯作者: 张慧勇 E-mail:beanphq@ciac.jl.cn

Microcantilever Biosensor on the Biotin-avidin Interaction

Zhang Huiyong1,2 Pan Hongqing1,2 Tang Jilin*,1 Zhang Bailin*,1   

  1. (1 State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022)
    (2 Graduate University of Chinese Academy of Sciences, Beijing 100049)
  • Received:2010-03-09 Revised:2010-06-29 Published:2010-08-16

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1406

被引次数

6

将生物素共价键合到微悬臂的硅表面, 利用微悬臂传感技术在流动注样条件下研究了生物素-亲和素的动态作用过程, 同时利用原子力显微镜(AFM)力刻蚀技术验证了生物素化硅表面结合亲和素的有效性. 结果显示亲和素与生物素作用产生较大的表面应力驱动微悬臂偏转, 悬臂偏转的程度和速率与亲和素的浓度相关, 亲和素浓度越大, 微悬臂偏转程度也越大, 同时反应速率也更快. 这项工作为开发新型的无标记检测技术, 研究生物分子之间相互作用提供了有益的探索.

关键词: 微悬臂, 生物素, 亲和素, 相互作用

The dynamic interaction process of biotin-avidin was investigated by biotin covalently modified microcantilever sensor in real-time in a flowing cell. The specific interaction was confirmed by the evidence that the avidin layer can be mechanically removed with atomic force microscopy nanolithography. The deflection of the modified cantilever increased with concentration of avidin. Microcantilever biosensor provides a good method to explore novel label-free technology and monitor various interactions between biomolecules.

Key words: microcantilever, biotin, avidin, interaction