Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (06): 803-811.DOI: 10.6023/A1107154 Previous Articles     Next Articles

Special Topic

不同功能单体合成的谷胱甘肽分子印迹聚合物的研究

辛瑜, 仝艳军, 杨海麟, 张玲, 张玉然, 陈亦, 王武   

  1. 江南大学生物工程学院工业生物技术教育部重点实验室 无锡 214122
  • 收稿日期:2011-07-15 修回日期:2011-11-08 出版日期:2012-03-28 发布日期:2011-12-14
  • 通讯作者: 王武
  • 基金资助:

    2010 年江苏省科技支撑(No. SBE201077545)、2011 年江苏省科技支撑(No. SBE201170578)、工业微生物重点实验室开放课题(No. KLIB-KF201005)、江苏省高校优势学科建设工程资助项目和“111”引智(No. 111-2-06)资助项目.

Study on the Glutathione Molecularly Imprinted Polymers with Different Functional Monomers

Xin Yu, Tong Yanjun, Yang Hailin, Zhang Ling, Zhang Yuran, Chen Yi, Wang Wu   

  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122
  • Received:2011-07-15 Revised:2011-11-08 Online:2012-03-28 Published:2011-12-14
  • Supported by:

    Project Supported by the Program of the Science and Technology Support Plan of Jiangsu Province (Nos. SBE201077545, SBE201170578), the Program of the Key Laboratory of Industrial Biotechnology, Ministry of Education, China (No. KLIB-KF201005), A Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions, the 111 Project (No. 111-2-06).

Using glutathione (GSH) as template molecule, acrylamide (AM) and methacrylic acid (MAA) as functional monomer, different glutathione-molecularly imprinted polymers (GSH-MIPs) were developed and synthesized with molecularly imprinted technique. The effects for adsorption capacity, including molar ratio of template molecule-functional monomer, pH of loading buffer, adsorption equilibrium time and loading concentration were discussed in detail. The result demonstrated that the polymers with raw material mole ratios at 1:5 for GSH:AM, and 1:4 for GSH:MAA showed higher adsorption capacity, the optimal pH values for the adsorption of GSH-MIPAMs and GSH-MIPMAAs to GSH were 3.0 and 5.0, respectively; furthermore, the optimal GSH loading concentrations were 1.50~2.00 g/L for GSH-MIPAMs, and 1.00~ 1.50 g/L for GSH-MIPMAAs; additionally, the adsorption equilibrium time was 20 h for GSH-MIPAMs and 18 h for GSH-MIPMAAs. In addition, the binding of GSH analogues were also applied to the synthesized polymers, and the results reflected that GSH-MIPs showed low recognition to these analogues. This protocol was further employed for the extraction and separation of GSH from yeast cells samples. The extraction recoveries of GSH-MIPAMs and GSH-MIPMAAs were 41% and 77%, respectively, it was of great potential in the purification of GSH.

Key words: molecularly imprinting, glutathione, GSH-MIPs, preparation, adsorption conditions, yeast extracts, adsorption performance