Acta Chimica Sinica ›› 2012, Vol. 0 ›› Issue (05): 617-623 .DOI: 10.6023/A1107302 Previous Articles     Next Articles

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郑静a,b, 赵家昌a, 龚晨a, 刘萍a, 陈瑜a, 黄焕a, 何品刚b, 方禹之b   

  1. a 上海工程技术大学化学化工学院 上海 201620;
    b 华东师范大学化学系 上海 200062
  • 投稿日期:2011-07-30 修回日期:2012-01-12 发布日期:2012-03-17
  • 通讯作者: 郑静
  • 基金资助:

    国家自然科学基金(No. 20675031)和上海市教委(No. 09YZ374)资助项目.

Preparation of the Ordered Mesoporous Carbon/Fe3O4 and Its Application for the Direct Electrochemistry of Hemoglobin

Zheng Jinga,b, Zhao Jiachanga, Gong Chena, Liu Pinga, Chen Yua, Huang Huana, He Pingangb, Fang Yuzhib   

  1. a College of Chemistry and Chemical Engineering, Shanghai University of Engineering Science, Shanghai 201620;
    b Department of Chemistry, East China Normal University, Shanghai 200062
  • Received:2011-07-30 Revised:2012-01-12 Published:2012-03-17
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 20675031) and Innovation Program of Shanghai Municipal Education Commission (No. 09YZ374).

The novel magnetic composites of ordered mesoporous carbon/Fe3O4 were prepared with a micro-wetness impregnation method. The composites were characterized by transmission electron microscope and X-ray diffraction. Hemoglobin was immobilized onto the surface of the composites, and was shown to keep its biological activity well. The electrochemical behavior of Hb was studied, and a pair of well-defined, quasi-reversible cyclic voltammetric redox peaks was obtained, which was characteristics of Hb Fe(III)/Fe(II) redox couples. The formal potential (E0'), the electron transfer number (n), and transfer coefficient (α) were calculated to be -0.306 V, 1.226 and 0.51, respectively. The average apparent heterogeneous electron transfer rate constant (KS) obtained for Hb was 0.0144 s-1. Linear sweep voltammetric measurement of Hb indicated that the peak currents of Hb were linear to its concentration ranging from 3.00×10-6 to 1.50×10-4 mol/L with the detection limits of 0.270×10-6 mol/L.

Key words: ordered mesoporous carbon, ferriferrous oxide, preparation, hemoglobin, direct electrochemistry