Acta Chimica Sinica ›› 2013, Vol. 71 ›› Issue (11): 1459-1476.DOI: 10.6023/A13070689 Previous Articles     Next Articles



楚宁宁, 冯成亮, 吉民   

  1. 东南大学化学化工学院 南京 211189
  • 投稿日期:2013-07-01 发布日期:2013-08-14
  • 通讯作者: 吉民
  • 作者简介:楚宁宁, 东南大学化学化工学院硕士, 目前在苏州东南药物研发有限责任公司实习.吉民, 东南大学化学化工学院教授、博士生导师、东南大学制药工程研究所所长, 东南大学化学化工学院制药工程系主任. 江苏省“青蓝工程”学术带头人培养对象、江苏省“333工程”培养对象、教育部优秀教师奖励计划资助对象.
  • 基金资助:

    项目受国家基础研究973 (No. 2011CB9335073)资助.

Advances in Applications of Fluorescent Probes Based on Indole Heptamethine in Vivo

Chu Ningning, Feng Chengliang, Ji Min   

  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189
  • Received:2013-07-01 Published:2013-08-14
  • Supported by:

    Project supported by National Basic Research 973 Project (No. 2011CB9335073).

Cyanine dye is a commercially available near-infrared fluorescent dye, its spectral range is in the near infrared region, in this spectrum, absorption or fluorescence intensity of biological sample is very small, so applied cyanine dye to image living body can reduce background interference. Indole heptamethine fluorescent probes which are representative of cyanine dyes are consisted of indole heterocycle, heptamethine and N-substituted side chains. They are widely used for targeted cancer therapy, labling protein, detecting of metal cations and other biological aspects because of their excellent photophysical properties like good solubility, tunable maximum absorption wavelength, large molar extinction coefficient. Based on the structure of indole heptamethine, introducing reactive groups to the parent compounds or changing their structures can make fluorescent probes have different functions like labeling protein and tumour, detecting intracellular metal cations, which has become the hotspot in the field of fluorescence imaging of biological research. Therefore in this paper, the recent progress in the field of applications of indole heptamethine based fluorescent probes for detecting metal cations, changes of pH, molecules and imaging cancer, protein in vivo are reviewed. Besides, the distribution, imaging results and metabolism of probes in vivo (model) have also been elaborated. The biological application trends and problems of this kind of fluorescent probes are also discussed in the end.

Key words: indole heptamethine, fluorescent, probe, biological, application