Biocompatible Phospholipid Modified Graphene Nanocomposite for Direct Electrochemistry of Redox Enzyme

doi:10.6023/A13080911

Acta Chimica Sinica ›› 2014, Vol. 72 ›› Issue (3): 388-394.DOI: 10.6023/A13080911 Previous Articles Next Articles

Special Issue: 石墨烯

Article

磷脂修饰化的石墨烯纳米复合物的制备及其直接电化学研究

张谦^a,b, 吴抒遥^a,b, 张玲^c, 茆卉^a,b, 刘大亮^a,b, 刘洋^d, 曾向群^e, 宋溪明^a,b, 李景虹^d

a 辽宁大学绿色合成与先进材料制备化学辽宁省重点实验室沈阳 110036;
b 辽宁大学化学院沈阳 110036;
c 沈阳师范大学化学与生命科学学院沈阳 110034;
d 清华大学化学系北京 100084;
e 奥克兰大学化学系罗切斯特市美国

投稿日期:2013-08-30 发布日期:2013-11-14
通讯作者: 李景虹，E-mail：jhli@mail.tsinghua.edu.cn；宋溪明，E-mail：songlab@lnu.edu.cn E-mail:jhli@mail.tsinghua.edu.cn；songlab@lnu.edu.cn
基金资助:
项目受国家自然科学基金（Nos. 21235004，21128005，51273087，21071070，21203126，21005046，51203072，20901035）；辽宁省高等学校创新团队基金（No. LT2011001）；辽宁省自然科学基金（Nos. 201202088，LJQ2013112）；辽宁省博士科研启动基金项目（No. 20131042）；辽宁大学青年科研基金（Nos. 2012LDQN08，2012LDQN07）；辽宁大学创新创业训练计划项目（No. X201210140036）和辽宁大学创新人才培养基金资助.

Biocompatible Phospholipid Modified Graphene Nanocomposite for Direct Electrochemistry of Redox Enzyme

Zhang Qian^a,b, Wu Shuyao^a,b, Zhang Ling^c, Mao Hui^a,b, Liu Daliang^a,b, Liu Yang^d, Zeng Xiangqun^e, Song Ximing^a,b, Li Jinghong^d

a Liaoning Provincial Key Laboratory for Green Synthesis and Preparative Chemistry of Advanced Materials, Liaoning University, Shenyang 110036;
b College of Chemistry, Liaoning University, Shenyang 110036;
c College of Chemistry and Life Science, Shenyang Normal University, Shenyang 110034;
d Department of Chemistry, Tsinghua University, Beijing 100084;
e Department of Chemistry, Oakland University, Rochester USA

Received:2013-08-30 Published:2013-11-14
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21235004, 21128005, 51273087, 21071070, 21203126, 21005046, 51203072, 20901035), the Financial Supports from the Program for Liaoning Innovative Research Team in University (No. LT2011001), the Natural Science Foundation of Liaoning Province (Nos. 201202088, LJQ2013112), the Research Fund for the Doctoral Program of Liaoning Province (No. 20131042), the Foundation for Young Scholars of Liaoning University (Nos. 2012LDQN08, 2012LDQN07), College students' innovative entrepreneurial training program (No. X201210140036) and the Foundation of 211 Project for Innovative Talents Training, Liaoning University.

A novel lipid based carbonaceous nanocomposite, 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-(1'-rac-glycerol) (sodium salt) (POPG) modified graphene (GP) (POPG-GP), was designed and synthesized by a non-covalent method. The nanocomposite was endowed with excellent properties of the two independent components, such as the biocompatibility of POPG and the outstanding electric properties of graphene. Fourier transform infrared (FT-IR) spectra, ultraviolet-visible (UV-vis) absorption spectra, transmission electron microscopy (TEM) were utilized to characterize the structure, morphology and surface property of the as synthesized POPG-GP. It has been found that the modification of POPG on GP could not only assist the dispersion of graphene in aqueous solution, but also endow it with negatively charged, which was favorable for the further immobilization of model enzyme via self-assembly. Based on the electrostatic interaction, the positively charged horseradish peroxide (HRP) could be immobilized onto the surface of POPG-GP to form HRP/POPG-GP/GC electrode. UV-vis and FT-IR spectroscopies were used to monitor the assembly process and demonstrated that HRP had been immobilized without denaturation. The HRP/POPG-GP/GC electrode could commendably realize the direct electron transfer (DET) between electrode and redox enzyme with good electrochemical performance. Moreover, such modified electrode also showed good electrocatalytic response toward the detection of H₂O₂ with high sensitivity, wide linear range, excellent stability and reproducibility. The linear response range for the HRP/POPG-GP/GC was 3.5～210 μmol/L (R=0.999). The detection limit and the sensitivity of the HRP/POPG-GP/GC electrode was calculated to be 1.17 μmol/L (S/N=3) and 356.6 mA·cm^-2·M^-1, respectively. The apparent Michaelis-Menten constant K_m was estimated to be 0.45 mmol/L, indicating a high affinity of HRP to H₂O₂ on POPG-GP. The experiment results demonstrated that POPG-GP not only provided a biocompatible microenvironment for the immobilized HRP, but also supplied a necessary pathway for its direct electron transfer. Therefore, such biocompatible nanocomposite had potential applications in the field of biosensors.

Key words: graphene, phospholipid, nanocomposite, horseradish peroxide, direct electrochemistry

Cite this article

Zhang Qian, Wu Shuyao, Zhang Ling, Mao Hui, Liu Daliang, Liu Yang, Zeng Xiangqun, Song Ximing, Li Jinghong. Biocompatible Phospholipid Modified Graphene Nanocomposite for Direct Electrochemistry of Redox Enzyme[J]. Acta Chimica Sinica, 2014, 72(3): 388-394.

Export EndNote|Reference Manager|ProCite|BibTeX|RefWorks

share this article

References

[1] Kang, X.; Wang, J.; Wu, H.; Aksay, I. A.; Liu, J.; Lin, Y. Biosens. Bioelectron. 2009, 25, 901.

[2] Liu, Y.; Wang, M.; Zhao, F.; Xu, Z.; Dong, S. Biosens. Bioelectron. 2005, 21, 984.

[3] Chen, H.; Dong, S. Biosens. Bioelectron. 2007, 22, 1811.

[4] Lu, X.; Hu, J.; Yao, X.; Wang, Z.; Li, J. Biomacromolecules 2006, 7, 975.

[5] Dai, Z.; Ju, H. Acta Phys.-Chim. Sin. 2004, 20, 1262. (戴志晖, 鞠熀先, 物理化学学报, 2004, 20, 1262.)

[6] Lu, X.; Zhang, Q.; Zhang, L.; Li, J. Electrochem. Commun. 2006, 8, 874.

[7] Zhang, Q.; Wu, S.; Zhang, L.; Lu, J.; Verproot, F.; Liu, Y.; Xing, Z.; Li, J.; Song, X.-M. Biosens. Bioelectron. 2011, 26, 2632.

[8] Cai, C.; Chen, J. Acta Chim. Sinica 2004, 62, 335. (蔡称心, 陈静, 化学学报, 2004, 62, 335.)

[9] Novoselov, K. S.; Geim, A. K.; Morozov, S. V.; Jiang, D.; Katsnelson, M. I.; Grigorieva, I. V.; Dubonos, S. V.; Firsov, A. A. Nature 2005, 438, 197.

[10] Li, D.; Muller, M. B.; Gilje, S.; Kaner, R. B.; Wallace, G. G. Nat. Nanotechnol. 2008, 3, 101.

[11] Zhang, Q.; Yang, S.; Zhang, J.; Zhang, L.; Kang, P.; Li, J.; Xu, J.; Zhou, H.; Song, X.-M. Nanotechnology 2011, 22, 494010.

[12] Zhang, Q.; Wu, S.; He, M.; Zhang, L.; Liu, Y.; Li, J.; Song, X.-M. Acta Chim. Sinica 2012, 70, 2213. (张谦, 吴抒遥, 何茂伟, 张玲, 刘洋, 李景虹, 宋溪明, 化学学报, 2012, 70, 2213.)

[13] Niyogi, S.; Bekyarova, E.; Itkis, M. E.; McWilliams, J. L.; Hamon, M. A.; Haddon, R. C. J. Am. Chem. Soc. 2006, 128, 7720.

[14] Yang, H.; Zhang, Q.; Shan, C.; Li, F.; Han, D.; Niu, L. Langmuir 2010, 26, 6708.

[15] Dayani, Y.; Malmstadt, N. Langmuir 2012, 28, 8174.

[16] Hummers, W. S.; Offeman, R. E. J. Am. Chem. Soc. 1958, 80, 1339.

[17] Wang, Z.; Li, M.; Su, P.; Zhang, Y.; Shen, Y.; Han, D.; Ivaska, A.; Niu, L. Electrochem. Commun. 2008, 10, 306.

[18] Li, J.; Dong, S. J. Electroanal. Chem. 1997, 431, 19.

[19] Zhang, L.; Zhang, Q.; Lu, X.; Li, J. Biosens. Bioelectron. 2007, 23, 102.

[20] Bond, A. M.; Braun, R. D. J. Electrochem. Soc. 1980, 127, 528C.

[21] Zhang, Q.; Qiao, Y.; Hao, F.; Zhang, L.; Wu, S.; Li, Y.; Li, J.; Song, X. M. Chem. Eur. J. 2010, 16, 8133.

[22] Mohammed, E.; Ignacio, N.-R.; Manuel, D.; Maria, P. H.-A.; Dolores, B.-M.; Jose, L. H.-H. C. Electrochim. Acta 2008, 53, 7131.

[23] Kamin, R. A.; Wilson, G. S. Anal. Chem. 1980, 52, 1198.

[24] Zhao, X.; Mai, Z.; Kang, X.; Zou, X. Biosens. Bioelectron. 2008, 23, 1032.

[25] Zhou, K.; Zhu, Y.; Yang, X.; Luo, J.; Li, C.; Luan, S. Electrochim. Acta 2010, 55, 3055.

磷脂修饰化的石墨烯纳米复合物的制备及其直接电化学研究

Biocompatible Phospholipid Modified Graphene Nanocomposite for Direct Electrochemistry of Redox Enzyme

PDF

Knowledge

Cited

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	Huagao Wang, Qunfeng Cheng. Recent Advances in the Nacre-inspired Layered Polymer Nanocomposites by Ice Templating Technique^★ [J]. Acta Chimica Sinica, 2023, 81(9): 1231-1239.
[2]	Congcong Ning, Qian Yang, Amin Mao, Zijia Tang, Yan Jin, Baoshan Hu. Research Progress in Controllable Preparation of Graphene Nanoribbons [J]. Acta Chimica Sinica, 2023, 81(4): 406-419.
[3]	Wen Liu, Yujie Wang, Huiqin Yang, Chengjie Li, Na Wu, Yang Yan. The Preparation of Carbon Nanotubes/Reduced Graphene Oxide Current Collector by Non-covalent Induction of Ionic Liquid for Sodium Metal Anode [J]. Acta Chimica Sinica, 2023, 81(10): 1379-1386.
[4]	Shaobing Yan, Long Jiao, Chuanxin He, Hailong Jiang. Pyrolysis of ZIF-67/Graphene Composite to Co Nanoparticles Confined in N-Doped Carbon for Efficient Electrocatalytic Oxygen Reduction [J]. Acta Chimica Sinica, 2022, 80(8): 1084-1090.
[5]	Xusheng Wang, Xu Yang, Chunhui Chen, Hongfang Li, Yuanbiao Huang, Rong Cao. Graphene Quantum Dots Supported on Fe-based Metal-Organic Frameworks for Efficient Photocatalytic CO₂ Reduction^※ [J]. Acta Chimica Sinica, 2022, 80(1): 22-28.
[6]	Yao Zhai, Guoxiang Xin, Jiaqi Wang, Bangwen Zhang, Jinling Song, Xiaoxu Liu. Microwave-assisted Synthesis of rGO/CeO₂ Supercapacitor Electrode Materials with Excellent Electrochemical Properties [J]. Acta Chimica Sinica, 2021, 79(9): 1129-1137.
[7]	Chang-An Liu, Shi-Bo Hong, Bei Li. Molecular Dynamics Simulation of the Stability Behavior of Graphene in Glycerol/Urea Solvents in Liquid-Phase Exfoliation [J]. Acta Chimica Sinica, 2021, 79(4): 530-538.
[8]	Jie Huang, Jiangbo Xi, Wei Chen, Zhengwu Bai. Graphene-derived Materials for Metal-free Carbocatalysis of Organic Reactions [J]. Acta Chimica Sinica, 2021, 79(11): 1360-1371.
[9]	Hua Yue, Guanghui Ma. Advances in Functionalized Carriers Based on Graphene's Unique Biological Interface Effect [J]. Acta Chimica Sinica, 2021, 79(10): 1244-1256.
[10]	Ma Minghao, Xu Ming, Liu Sijin. Surface Chemical Modifications of Graphene Oxide and Interaction Mechanisms at the Nano-Bio Interface [J]. Acta Chimica Sinica, 2020, 78(9): 877-887.
[11]	Li Haimei, Luo Huajian, Xiao Qi, Yang Liyun, Huang Shan, Liu Yi. Investigations of Interactions and Mechanisms of Chiral Graphene Quantum Dots with DNA [J]. Acta Chimica Sinica, 2020, 78(6): 577-586.
[12]	Sun Yanhui, Qi Youxiao, Shen You, Jing Cuijie, Chen Xiaoxiao, Wang Xinxing. Preparation of Electrochemical Sensor Based on RGO-Au-ZIF-8 Composite and Its Application in Simultaneous Detection of Lead Ions and Copper Ions [J]. Acta Chimica Sinica, 2020, 78(2): 147-154.
[13]	Zhao Yajing, Xie Liang, Ma Lanchao, He Junhui. Preparation and Application of Polydimethylsiloxane Encapsulated Graphene-based Flexible Infrared Detector [J]. Acta Chimica Sinica, 2020, 78(2): 161-169.
[14]	Song Guangjie, Wu Tiaodi, Liu Fuxin, Zhang Binyan, Liu Xiuhui. Electrochemical Detection of Xanthine and Study for the Inhibition of Uric Acid Based on Chitosan/Nitrogen Doped Reduced Graphene Oxide Modified Electrode [J]. Acta Chimica Sinica, 2020, 78(1): 82-88.
[15]	Guo Yu, Li Yanrui, Wang Chengming, Long Ran, Xiong Yujie. Photogenerated Charge Separation and Photocatalytic Hydrogen Production of TiO₂/Graphene Composite Materials [J]. Acta Chim. Sinica, 2019, 77(6): 520-524.