Acta Chimica Sinica ›› 2021, Vol. 79 ›› Issue (3): 338-343.DOI: 10.6023/A20090422 Previous Articles     Next Articles



吴峰a,b, 苏倩倩a,b, 周乐乐a,b, 许鹏飞a,b, 董傲a,b, 钱卫平a,b,*()   

  1. a 东南大学生物电子学国家重点实验室 南京 210096
    b 东南大学生物科学与医学工程学院 南京 210096
  • 投稿日期:2020-09-13 发布日期:2021-02-05
  • 通讯作者: 钱卫平
  • 作者简介:
  • 基金资助:
    项目受国家自然科学基金(21775020); 国家重点研发计划(2017YFE0100200); 国家重点研发计划(2017YFA0205303)

A Novel Protein Corona Characterization based on the Reflectometric Interference Spectroscopy with Silica Colloidal Crystal Films

Feng Wua,b, Qianqian Sua,b, Lele Zhoua,b, Pengfei Xua,b, Ao Donga,b, Weiping Qiana,b,*()   

  1. a State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China
    b School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, China
  • Received:2020-09-13 Published:2021-02-05
  • Contact: Weiping Qian
  • Supported by:
    National Natural Science Foundation of China(21775020); National Key Research and Development Program of China(2017YFE0100200); National Key Research and Development Program of China(2017YFA0205303)

It will form protein corona on the surface of nanoparticles when exposed to the biological environment. The process of protein corona formation is crucial in biomedical engineering, nanomaterials development and research, especially the protein corona formation process of medical engineering materials in blood samples, and there is no mature method for real- time monitoring. Silica colloidal crystal films were prepared by vertical deposition on the glass slides. The glass slides were placed vertically in an ethanol suspension of silica nonospheres with diameters of 110, 190 and 280 nm. After 7 days of ethanol evaporation, a large area of silica colloidal crystal film was formed on the surface of the glass slide. The glass slides of silica colloidal crystal films were assembled into reaction cell, and the reflectometric interference spectroscopy (RIfS) system assembled by microscope light source and spectrometer were used for detection and optical thickness as a parameter. And different concentrations of hydrochloric acid were used to test the performance of the RIfS system. In this study, RIfS was utilized to detect the protein corona formation of human serum albumin, human fibrinogen and immunoglobulin G, as well as blood samples by silica colloidal crystal films which have special optical properties. It was found that the formation of immunoglobulin G had the highest optical thickness variation due to its conformation. According to the formation process of human serum albumin on silica nanosheres with different diameters, the optical thickness of protein corona was also higher on the of silica nanospheres with higher curvature. In addition, through the detection of plasma and whole blood samples, it was found that the silica colloidal crystal films could basically eliminate the interference of blood cells to the detection of protein corona. This work developed a real-time unlabeled protein corona research method, which provides a basis for the clinical application of biomedical materials.

Key words: plasma protein, protein corona, reflectometric interference spectroscopy, silica colloidal crystal film, ordered porous layer interferometry