Acta Chimica Sinica ›› 2022, Vol. 80 ›› Issue (5): 625-632.DOI: 10.6023/A21120606 Previous Articles     Next Articles

Article

模板法控制合成AgInSe2:Zn2+近红外荧光量子点及其生物标记应用

廉纬a,b, 方泽铠a,b, 涂大涛a,b,c,*(), 李嘉尧a,b, 韩思远b, 李仁富b,c, 商晓颖b,c, 陈学元a,b,c,*()   

  1. a 福州大学 化学学院 福州 350108
    b 中国科学院福建物质结构研究所 中国科学院功能纳米结构设计与组装重点实验室 福州 350002
    c 中国科学院福建物质结构研究所 结构化学国家重点实验室 福州 350002
  • 投稿日期:2021-12-30 发布日期:2022-05-31
  • 通讯作者: 涂大涛, 陈学元
  • 作者简介:
    庆祝中国科学院青年创新促进会十年华诞.
  • 基金资助:
    国家自然科学基金(U1805252); 国家自然科学基金(21975257); 国家自然科学基金(22135008); 福建省自然科学基金(2019I0029); 福建省自然科学基金(2021L3024)

Template-Based Controlled Synthesis and Bioapplication of AgInSe2:Zn2+ Near-Infrared Luminescent Quantum Dots

Wei Liana,b, Zekai Fanga,b, Datao Tua,b,c(), Jiayao Lia,b, Siyuan Hanb, Renfu Lib,c, Xiaoying Shangb,c, Xueyuan Chena,b,c()   

  1. a College of Chemistry, Fuzhou University, Fuzhou 350108, China
    b CAS Key Laboratory of Design and Assembly of Functional Nanostructures, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China
    c State Key Laboratory of Structural Chemistry, Fujian Institute of Research on the Structure of Matter, Chinese Academy of Sciences, Fuzhou 350002, China
  • Received:2021-12-30 Published:2022-05-31
  • Contact: Datao Tu, Xueyuan Chen
  • About author:
    Dedicated to the 10th anniversary of the Youth Innovation Promotion Association, CAS.
  • Supported by:
    National Natural Science Foundation of China(U1805252); National Natural Science Foundation of China(21975257); National Natural Science Foundation of China(22135008); Natural Science Foundation of Fujian Province(2019I0029); Natural Science Foundation of Fujian Province(2021L3024)

AgInSe2 (AISe) quantum dots (QDs) exhibit large Stokes shift, composition-dependent photoluminescence (PL), long PL lifetimes and low toxicity, making them exceptional candidates in a wide variety of bioapplications. However, it remains notoriously challenging to precisely control both the morphology and composition to optimize the PL performance of AISe QDs via conventional direct synthesis. Herein, we develop the unique low-temperature (75 ℃) template-based synthesis of highly efficient near-infrared (NIR) luminescent AISe QDs from In2Se3 QDs via a facile cation exchange method. The brief synthesis AISe QDs process was as follows: firstly, indium acetate was dissolved in non-coordinating solvent octadecene. Selenium precursor was injected into the above mixture at 200 ℃, followed by nucleation and growth within a few minutes. Thereafter, In2Se3 template QDs can be acquired, and the dispersity of the as-prepared QDs can be improved by adding zinc. Secondly, silver acetate was added to the In2Se3:Zn2+ QDs solution with stirring for 15 min at 75 ℃. Finally, AgInSe2:Zn2+ QDs were obtained. The proposed method enables the as-prepared AISe QDs to inherit the size and morphology of the template QDs. The extent of cation exchange can be controlled by rationally manipulating the Ag/In precursor molar ratio. We successfully regulate the stoichiometry of Ag/In ratio from 0.26 to 1.09. As a result, highly efficient luminescence of AISe QDs with the maximum absolute quantum yield of 42.5% has been achieved, which is higher than that of the AISe counterparts synthesized via the direct method. Moreover, we survey the luminescence mechanism of AISe QDs by means of the steady-state, transient and temperature-dependent spectroscopies. AISe nanoprobes were prepared by coating the hydrophobic QDs with a layer of 1,2-distearoyl-sn-glycero-3-phosphoethanol-amine-N-[biotin(polyethyleneglycol)- 2000] (DSPE-PEG-Biotin) phospholipids through hydrophobic interaction. By virtue of the excellent biocompatibility and intense NIR emission, we exemplify the application of AISe nanoprobes in the targeted cancer cell imaging, thus revealing their promising bioapplications including disease diagnosis and imaging-guided surgery.

Key words: AgInSe2, near-infrared, quantum dots, cation exchange, luminescent nanoprobes