Acta Chimica Sinica ›› 2012, Vol. 70 ›› Issue (20): 2123-2126.DOI: 10.6023/A12090619 Previous Articles     Next Articles



张良, 于淼, 何川   

  1. 化学系及生物物理动态研究中心 芝加哥大学 芝加哥 美国 60637
  • 投稿日期:2012-09-03 发布日期:2012-09-19
  • 通讯作者: 何川;

Mouse Tet1 Protein can Oxidize 5mC to 5hmC and 5caC on Single-stranded DNA

Zhang Liang, Yu Miao, He Chuan   

  1. Department of Chemistry and Institute for Biophysical Dynamics, The University of Chicago, 929 E. 57th Street, Chicago, Illinois 60637, USA
  • Received:2012-09-03 Published:2012-09-19

5-Methylcytosine (5mC) is known to play broad roles in mammalian biology. It can be enzymatically oxidized to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), and 5-carboxylcytosine (5caC) by a family of iron(II)/αKG-dependent dioxygenases Tet proteins. This process has been shown to play critical roles in mammalian cell development and dynamic methylation regulation. Previous research has shown that Tet proteins can oxidize 5mC on double-stranded DNA (dsDNA). Here, we expressed and purified the active domain of mouse Tet1 DNA from insect cell. By employing mass spectrometry, we show here that mtet1 (1367—2039) can also oxidize 5mC on single-stranded DNA (ssDNA), thus indicating its potential involvement in 5mC oxidation during replication or/and transcription, as well as the oxidation of corresponding modifications on single-stranded RNA in mammalian cells.

Key words: Tet, 5-methylcytosine (5mC), 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC), 5-carboxylcytosine (5caC), double-stranded DNA (dsDNA), single-stranded DNA (ssDNA), oxidation