有机化学 ›› 2014, Vol. 34 ›› Issue (10): 2135-2139.DOI: 10.6023/cjoc201404025 上一篇    下一篇

研究简报

爆炸条件下溶菌酶反应产物的基质辅助激光解吸-飞行时间质谱分析

刘素红a, 夏攀b, 张成功b, 张立a, 郭寅龙a   

  1. a. 中国科学院上海有机化学研究所上海质谱中心 上海 200032;
    b. 上海市现场物证重点实验室 上海 200042
  • 收稿日期:2014-04-15 修回日期:2014-05-26 出版日期:2014-10-25 发布日期:2014-06-11
  • 通讯作者: 张成功, 张立 E-mail:zhangli7488@sioc.ac.cn
  • 基金资助:

    上海市现场物证重点实验室开放课题(No. 2012XCWZK07)及国家自然科学基金(Nos. 21202192, 21275155)资助项目.

Analysis of Reaction Products of Lysozyme under the Explosion Condition by Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry

Liu Suhonga, Xia Panb, Zhang Chenggongb, Zhang Lia, Guo Yinlonga   

  1. a. Shanghai Mass Spectrometry Center, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032;
    b. Shanghai Institute of Forensic Science, Shanghai 200042
  • Received:2014-04-15 Revised:2014-05-26 Online:2014-10-25 Published:2014-06-11
  • Supported by:

    Project supported by the Open Project of Shanghai Institute of Forensic Science (No. 2012XCWZK07) and the National Natural Science Foundation of China (Nos. 21202192, 21275155).

以爆炸条件下溶菌酶的反应产物为研究对象,运用基质辅助激光解吸-飞行时间质谱的方法研究其反应产物与不同炸药的反应特征性. 结果显示,雷管的使用不会对溶菌酶有影响,而在有爆炸物爆炸的条件下,除了正常溶菌酶酶解多肽信号外,一些[MH+17]+,[MH+18]+,[MH+28]+,[MH+32]+和[MH+44]+的加合峰信号也能被检测到. 这可能是在炸药爆炸过程中产生的一些活性小分子(如NH3,H2O,CO/N2,O2,CO2)与溶菌酶发生反应得到的反应产物. 不同炸药生成的活性小分子能够分别与溶菌酶的某些反应位点反应生成特征性的反应产物,有利于这些炸药的分析与检测.

关键词: 爆炸物, 基质辅助激光解吸-飞行时间质谱, 梯恩梯(TNT), 黑索今(RDX), 太恩(PETN), 黑火药, 烟火药, 硝铵

Identification and determination of explosives and explosive residues were a subject of continuing strong interest in analytical chemistry and forensic science. In this paper, the reaction products of lysozyme under the explosion condition were analyzed by a MALDI-TOFMS (Matrix-assisted laser desorption ionization time-of-flight mass spectrometry) method. There was no difference in the tryptic digest between the normal lysozyme and the reaction products generated by detonator, while some adduct peaks such as [MH+17]+, [MH+18]+, [MH+28]+, [MH+32]+, and [MH+44]+ were discovered in the explosives. This may be attributed to the reaction between the lysozyme and the active small molecule gases such as NH3, H2O, CO/N2, O2, CO2, which were generated during the explosion. Characteristic peaks which were produced by lysozyme and the active small molecule gases from different explosives can be used to discriminate the six explosives. For example, H2O molecules which were generated during the exploration by tri-nitrotoluene (TNT) can specifically react with VFGRCELAAAMKRHGLDNYR (m/z 2307) to produce a characteristic peak at m/z 2325 (2307+18). Also, H2O molecules which were generated by hexahydro-1,3,5-trinitroazine (RDX) can completely react with IVSDGNGMNAWVAWRNRCK (m/z 2177) to produce a characteristic peak at m/z (2177+18). Characteristic peak at m/z 1301 was produced by GYSLGNWVCAAK (m/z 1269) and O2 molecules for the identification of pentaerythritol tetranitrate (PETN). While for black powder, O2 and H2O can both react with IVSDGNGMNAWVAWR (m/z 1676) to produce product ions peaks at m/z 1694 and 1708. However, only the O2 molecules can react with IVSDGNGMNAWVAWR for pyrotechnic composition. As for ammon explosive, which is a mixture of inorganic explosives and organic explosives, CO2 molecules can react with a plurality of reaction sites of lysozyme to produce a series of characteristic peaks signals such as m/z 1313 (1269+44), 1720 (1676+44), 1848 (1804+44), 2722 (2678+44).

Key words: explosive, MALDI-TOFMS, TNT, RDX, PETN, black powder, pyrotechnic composition, ammon explosive