Chin. J. Org. Chem. ›› 2015, Vol. 35 ›› Issue (12): 2629-2635.DOI: 10.6023/cjoc201506017 Previous Articles     Next Articles



李波, 孟文斐, 杨美盼, 苏娜, 刘晓静, 杨秉勤   

  1. 西北大学化学与材料科学学院 合成与天然功能分子化学教育部重点实验室 西安 710127
  • 收稿日期:2015-06-15 修回日期:2015-07-11 发布日期:2015-08-26
  • 通讯作者: 杨秉勤;
  • 基金资助:

    国家自然科学基金(No. 21172178)资助项目.

Novel Fluorescence Probe for Fe3+and Application of Imaging in Living Cells

Li Bo, Meng Wenfei, Yang Meipan, Su Na, Liu Xiaojing, Yang Bingqin   

  1. Key Laboratory of Synthetic and Natural Functional Molecule Chemistry of Ministry of Education, College of Chemistry & Materials Science, Northwest University, Xi'an 710069
  • Received:2015-06-15 Revised:2015-07-11 Published:2015-08-26
  • Supported by:

    Project supported by the National Natural Science Foundation of China (No. 21172178).

A novel rhodamine B derivative R1 was synthesized facilely by the condensation reaction of rhodamine B, 2-aminothiophenol and benzyl chloride, and the structure of R1 was further characterized by IR, HRMS-ESI, 1H NMR, 13C NMR. Then, the recognition properties of compound R1 with metal ions had been investigated in water solution by the fluorescence spectrophotometry. The results showed that probe R1 demonstrated excellent specificity toward Fe3+with high speed and sensitivity, and the fluorescence intensity was not affected by the other common metal ions. Furthermore, there was a good linearity between the fluorescence intensity and the concentration of Fe3+. Importantly, fluorescence imaging experiments of Fe3+ in living cells demonstrated their valuable application.

Key words: fluorescence probe, rhodamine B, Fe3+, recognition, PC-12 living cells