Chinese Journal of Organic Chemistry ›› 2020, Vol. 40 ›› Issue (4): 959-968.DOI: 10.6023/cjoc201909017 Previous Articles     Next Articles


王淑琴a, 黄婉云a, 张小蓉a, 张晓婷a, 潘成学b   

  1. a 桂林医学院药学院 广西桂林 541004;
    b 广西师范大学化学与药学学院 药用资源化学与药物分子工程国家重点实验室 广西桂林 541004
  • 收稿日期:2019-09-10 修回日期:2019-11-27 发布日期:2020-05-06
  • 通讯作者: 黄婉云
  • 基金资助:

Synthesis and Bioactive Evaluation of Pyridazino-[6,1-b]quinazolinones Derivatives

Wang Shuqina, Huang Wanyuna, Zhang Xiaoronga, Zhang Xiaotinga, Pan Chengxueb   

  1. a College of Pharmacy, Guilin Medical University, Guilin 541004;
    b State Key Laboratory for Chemistry and Molecular Engineering of Medicinal Resources, Guangxi Normal University, Guilin, Guangxi 541004
  • Received:2019-09-10 Revised:2019-11-27 Published:2020-05-06
  • Supported by:
    Project supported by the National Natural Science Foundation of China (No. 21362007).

Novel nitrogen-containing heterocyclic scaffold pyridazino[6,1-b]quinazolinones were designed by the combination of two privileged structure units. Then a scheme for the synthesis of pyridazino[6,1-b]quinazolinone derivatives in 4 steps starting from methyl 2-aminobenzoate was developed in which the intramolecular condensation of acylhydrazone with ester was a key step transformation. 14 pyridazino[6,1-b]quinazolinone derivatives were synthesized and their structures were characterized and comfirmed by 1H NMR, 13C NMR and HRMS. Their in vitro cytotoxic activities against a panel of human tumor cell lines (SK-OV-3, CNE-2, MGC-803, NCI-H460) and nomal liver cell LO2 were evaluated via methyl thiazolyl tetrazolium (MTT) assay. The result indicated that 2-(4-bromophenyl)-4-hydroxy-10H-pyridazino[6,1-b]quinazolin-10-one (4b) and 2-(3-chlorophenyl)-4-hydroxy-10H-pyridazino[6,1-b]quinazolin-10-one (4d) exhibted very potent cytotoxic activity. The IC50value of 4b against CNE-2 and 4d against NCI-H460 cell line lowed to 0.85 and 2.31 μmol/L, respectively, far potent than the positive control cisplatin and almost equivalent to the natrual anticancer medicine camptothecin. Ultraviolet spectrometry and fluorescence titration assay were carried out to evaluate the ability of the compound to interact with DNA. The result indicated that they were able to intercalate in to DNA. Compound 4d, which demonstrated potent antitumor activity while less cytotoxicity against the normal liver cell LO2, could arrest cell cycle at G1 phase and significantly induce apoptosis on NCI-H460 cell in a dose-dependent manner, while 4b which exhibit potent cytotoxicity against LO2, could result in significant DNA double strand break when treated with the cell.

Key words: quinazolinones, pyridazine, antitumor, interaction with DNA