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化学学报
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化学学报 ›› 1999, Vol. 57 ›› Issue (9): 981-985. 上一篇    下一篇

研究论文

金属卟啉MTPPS对NADH氧化的催化作用

吴宝璋;杨长通;吴辉煌   

  1. 厦门大学化学系.厦门(361005);厦门大学固体表面物理化学国家重点实验室.厦 门(361005)
  • 发布日期:1999-09-15

Catalytic oxidation of reduced coenzyme NADH by metalloporphrins MTPPS

Wu Baozhang;Yang Changtong;Wu Huihuang   

  1. Xiamen Univ, Dept Chem.Xiamen(361005);State Key Lab Phys Chem Solid Surface, Xiamen Univ, Dept Chem.Xiamen(361005)
  • Published:1999-09-15

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438

被引次数

4 | 2

中位-四(对-磺基苯基)卟啉的铁、锰配合物(FeTPPS和MnTPPS)用作NADH氧化的催化剂,均相溶液中的反应动力学用紫外可见光谱测定。结果表明,在除氧的中性溶液中FeTPPS和MnTPPS降低了NADH在玻碳电极上氧化的过电位,过程用EC再生机理解释。在氧饱和的溶液中MTPPS起电子转移中介体的作用促进NADH氧化,其还原态被O~2氧化而生。测得FeTPPS和NADH反应的速率常数为3.3mol^-^1.L.s^-^1,而MnTPPS和NADH反应的速率常数约为FeTPPS的1/2。讨论了MTPPS作为NADH仿生氧化催化剂的前景。

关键词: 卟啉, 铁, 锰, 络合物, 动力学分析, 生物催化, 辅酶, 氧化

The metalloporphrins FeTPPS and MnTPPS [TPPS standing for meso- tetrkis (p-sulfonatophenyl) porphyrin], were used as the catalysts for NADH oxidation. The reaction kinetics in solution was measured by UV- vis spectroscopy. It was found that in neutral deoxygenated solution, the presence of FeTPPS or MnTPPS decreases the overpotential of NADH oxidation at a glassy carbon electrode, the processes are explained in terms of EC mechanism. In oxygen-saturated solution, MTPPS as an electron-transfer mediator accelerated NADH oxidation, and reduced MTPPS was regeneraed via chemical oxidation by O~2. The rate constant for the oxidation of NADH by FeTPPS was measured as 3.3mol^-^1.L.s^- ^1, however, for MnTPPS the rate constant was about a half of that for FeTPPS. The prospects of using MTPPS as biomimitic catalysts for NADH oxidation are discussed.

Key words: PORPHYRIN, IRON, MANGANESE, COMPLEX COMPOUNDS, KINETIC ANALYSIS, BIOCATALYSIS, COENZYME, OXIDATION

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