化学学报 ›› 2000, Vol. 58 ›› Issue (9): 1125-1130. 上一篇    下一篇

研究论文

DMPC与蛋白质在气-液界面上复合组装过程研究

张恒建;陈志坚;毕只初;王明强;翟秀红;崔光晨;李峻柏   

  1. 中国科学院化学研究所.北京(100080);中国科学院分子科学中心胶体与界面科 学实验室
  • 发布日期:2000-09-15

The self-assembly process of DMPC/protein at the air/water interface

Zhang Hengjian;Chen Zhijian;Bi Zhichu;Wang Mingqiang;Di Xiuhong;Cui Guangchen;Li Junbai   

  1. Inst of Chem, CAS.Beijing(100080)
  • Published:2000-09-15

对磷脂DMPC与蛋白质(β-lactoglobulin,β-casein或humanserumalbumin)组成的复合单分子膜在压缩过程中的相变进行了研究,同时通过Brewster角显微镜观察了磷脂与蛋白质在空气-水界面处发生的自组装过程。发现当低表面压时在DMPC/β-casein,DMPC/humanserumalbumin和DMPC/β-lactoglobulin复合单分子膜上分别出现线状和块状微区,而在较高表面压时只有DMPC/β-casein体系出现钩状的微区。说明微区的形貌与蛋白质的种类及构象变化有关。

关键词: 蛋白质, 磷脂, 单分子膜, 自组装, 相变, Brewster角显微镜

The phase changes of the complex monolayer composed of DMPC and β- lactoglobulin or β-casein during the compression process were investigated. The self-assembly process of the phospholipid and the protein at the air/water interface was observed via Brewster angle microscopy. It was found that at the low surface pressure during the compress process the linear or piece shape domain appeared with the DMPC/β-casein or the DMPC/β-lactoglobulin complex monolayer, respectively. At the higher surface pressure, the hook shape domains were formed for the DMPC/β-casein system.

Key words: PROTEIN, PHOSPHOLIPID, MONOMOLECULAR FILMS, PHASE TRANSFORMATION

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