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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (10): 1822-1827. 上一篇    下一篇

研究论文

乙醇敏化钛黄与蛋白质作用的共振光散射光谱研究及其分析应用

吴会灵;李文友;何锡文   

  1. 南开大学化学系.天津(300071)
  • 发布日期:2002-10-15

Study of Ethanol Sensitizing Effect on the Interaction of Titan Yellow and Proteins by Resonance Light Scattering Technology and Its Analytical Application

Wu Huiling;Li Wenyou;He Xiwen   

  1. Department of Chemistry, Nankai University.Tianjin(30071)
  • Published:2002-10-15

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539

被引次数

72 | 10

研究了在乙醇存在下钛黄（TY）与蛋白质作用的共振光散射（RLS）光谱特征 。基于RLS的增强，建立了一种测定蛋白质的新方法。考察了各种影响因素，在优 化条件下确定了RLS强度与蛋白质浓度之间的关系。当TY浓度为4 * 10~(-5)mol· L~(-1)时，牛血清白蛋白（BSA）、人血清白蛋白（HSA）和溶菌酶(lysozyme)的线 性响应范围分别为0.1～6.0 μg·mL~(-1), 0.1～5.0 μg·mL~(-1), 0.2～3.0 μg·mL~(-1)，检出限分别为12.7 ng·mL~(-1), 11.6 ng·mL~(-1)和18.8 ng· mL~(-1)。三种合成样品五次平行测定的回收率和相对标准偏差（RSD）分别为96. 0%～102.3%和0.8%～3.2%。将该方法与经典的Bradford方法分别用于人血清试样中 蛋白的测定，两种方法的分析结果经F-检验和双总体T-检验证明无显著性差异。

关键词: 钛黄, 蛋白质, 共振散射, 乙醇, 溶菌酶

The determination of proteins with tian yellow by resonance light scattering (RLS) technique was studied in the presence of ethanol. At pH = 4.1, a new method for the determination of proteins based on the enhancement of RLS has been developed. The linear ranges of the calibration graphs are 0.1～6.0 μg·mL~(-1) for bovine serum albumin (BSA), 0.1～5.0 μg·mL~(-1) for human serum albumin (HSA) and 0.2～3.0 μg·mL~(-1) for lysozyme, respectively. The corresponding determination limits are 12.7 ng·mL~(-1), 11.6 ng·mL~(-1) and 18.8 ng·mL~(-1), respectively. Synthetic samples and authentic samples were analyzed satisfactorily.

Key words: TITANIUM YELLOW, PROTEIN, PROTEIN, ETHANOL, LYSOZYME

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