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化学学报
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化学学报 ›› 2003, Vol. 61 ›› Issue (10): 1692-1696. 上一篇    下一篇

研究论文

羟乙基化牛膝多糖的合成及其活性研究

张健;田庚元   

  1. 中国科学院上海有机化学研究所
  • 发布日期:2003-10-15

Studies on Synthesis, Structure and Bioactivity of Hydroxyethylated Abps

Zhang Jian;Tian Gengyuan   

  1. Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences
  • Published:2003-10-15

PDF

488

被引次数

58 | 2

以环氧乙烷为羟乙基化试剂，在碱性水溶液中，对牛膝多糖进行羟乙基化，经 过丙酮沉淀、膜分离、Sephadex G-25柱层析等分离方法得到羟乙基化牛膝多糖纯 品，检测其理化性质，并通过甲基化、GC-MS分析，初步确证糖链中羟乙基主要取 代在葡萄糖6位和困糖的1位上。药理实验表明，羟乙基化牛膝多糖对荷Lewis肺癌 小鼠NK活性具有一定促进作用。

关键词: 牛膝, 羟乙基牛膝多糖, 结构, 抗肿瘤药, 环氧乙烷, 色谱-质谱, 药理

Achyranthes bidentata polysaccharides (Achyranthan, Abps) was hydroxyethylated by ethylene oxide in aqueous NaOH. The crude product was purified by acetone precipitation, dialysis, and Sephadex G-25 column chromatography to give hydroxyethylated Abps (Abps-HE), which was identified by some physicochemical properties. By the methylation method and GC-MS analysis, it is concluded that the hydroxyethyl groups are mainly substituted on 6-position of the glucopyranose and fructofuranose of the sugar backbone. The product possesses antitumor activity and improves NK cell activity in Lewis lung cancer mice.

Key words: achyranthes bidentata, hydroxyethylated abps, STRUCTURE, ANTITUMOR DRUGS, ETHYLENE OXIDE, CHROMATOGRAPHY-MASS SPECTROMETRY, PHARMACOLOGY

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