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化学学报
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化学学报 ›› 2005, Vol. 63 ›› Issue (4): 259-262.    下一篇

研究通讯

然聚电解质壳聚糖/羧甲基壳聚糖配合物膜的研制

杨前荣, 陈　新*, 邵正中   

  1. (复旦大学高分子科学系　教育部聚合物分子工程重点实验室　上海 200433)
  • 投稿日期:2004-09-13 修回日期:2004-11-10 发布日期:2010-12-10
  • 通讯作者: 陈　新

Preparation of Natural Polyelectrolytical Chitosan/Carboxymethyl Chitosan Complex Membrane

YANG Qian-Rong, CHEN Xin*, SHAO Zheng-Zhong   

  1. (Department of Macromolecular Science, Key Laboratory of Molecular Engineering of Polymers of Ministry of Education, Fudan University, Shanghai 200433)
  • Received:2004-09-13 Revised:2004-11-10 Published:2010-12-10
  • Contact: CHEN Xin

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制备了一种新型聚电解质膜壳聚糖/羧甲基壳聚糖配合物膜, 并对膜的结构进行了初步表征; 同时考察了不同配比的配合物膜对模型蛋白质溶菌酶的吸附性能. 结果表明, 在碱性条件下(pH＝9.2), 随着羧甲基壳聚糖含量的增加, 膜对溶菌酶的吸附能力也随之增强; 当羧甲基壳聚糖的含量为w＝40%时, 膜的吸附性能较佳, 可达92 mg/g.

关键词: 膜色谱, 共混, 多孔膜, 蛋白质, 吸附, 交联

A novel polyelectrolytical complex membrane between chitosan and carboxymethyl chitosan has been prepared and the primary structure characterization has been performed. Meantime, the lysozyme adsorption capacities on these complex membranes with various chitosan/carboxymethyl chitosan compositions have been tested. The results showed that the complex membranes had significant adsorption capacity to lysozyme and the adsorption capacity increased with the increase of carboxymethyl chitosan content in the membrane. When carboxymethyl chitosan content in the membrane is 40%, the lysozyme adsorption capacity can reach as high as 92 mg/g.

Key words: membrane chromatography, blend, macroporous membrane, protein, adsorption, crosslinking