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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (8): 738-742. 上一篇    下一篇

研究论文

基于去保护机理的新型H2O2荧光探针的制备及应用

吴朝阳*，范占煌，唐友云，沈国励，俞汝勤   

  1. (湖南大学化学化工学院 化学生物传感与计量学国家重点实验室 长沙 410082)
  • 投稿日期:2005-09-07 修回日期:2005-12-22 发布日期:2006-04-25
  • 通讯作者: 吴朝阳

Synthesis and Application of Novel H2O2 Fluorescent Probe Based on the Deprotection Mechanism

WU Zhao-Yang*, FAN Zhan-Huang, TANG You-Yun, SHEN Guo-Li, YU Ru-Qin   

  1. (College of Chemistry and Chemical Engineering, State Key Laboratory of Chemo-Biosensing and Chemometrics,
    Hunan University, Changsha 410082)
  • Received:2005-09-07 Revised:2005-12-22 Published:2006-04-25
  • Contact: WU Zhao-Yang

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合成的3',6'-对戊酰硼荧光素是一种基于去保护机理的高灵敏H2O2荧光增强化合物, 能灵敏地检测出样品中H2O2的含量. 用IR, 1H NMR 对该探针的结构进行了表征, 并且讨论了反应时间、溶剂、碱对探针合成的影响. 研究了探针的最佳响应时间, 结果表明检测过程中反应的最佳时间为3 min. 该探针在2.3×10－12～2.0×10－8 mol/L H2O2溶液范围内呈荧光增强趋势, 检测下限为2.3×10－12 mol/L. 此探针重现性良好, 在血清中检测H2O2的回收率在93.9%～102.2%之间, 结果令人满意, 具有一定的实用价值.

关键词: 3',6'-对戊酰硼荧光素, 荧光探针, H2O2, 去保护

The compound, 3',6'-bis(tetramethylethylenedioxyboryl)fluorane, synthesized in this experiment is a kind of H2O2 fluorescence-strengthened reagent, which depends on deprotection mechanism and can examine the H2O2 concentration sensitively. The structure of the compound was confirmed by the IR and 1H NMR spectra. The influences of reaction time, solvent and base on the probe synthesis were investigated in detail. The response time of the fluorescent probe was also studied and as a result, the optimal time for the H2O2 detection is 3 min. The fluorescence intensity of the probe was gradually increased with the increase of H2O2 concentration in the range of 2.3×10－12～2.0×10－8 mol/L, the detection limit of H2O2 was 2.3×10－12 mol/L, and the recovery rates in the blood serum were in the range of 93.9%～102.2%.

Key words: 3',6'-bis(tetramethylethylenedioxyboryl)fluorane, fluorescent probe, H2O2, deprotection