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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (14): 1740-1744. 上一篇    下一篇

研究论文

镧在轮藻节间细胞中的分布及与细胞壁的结合状态

姜文君a 李子杰a 张智勇*,a 张 静b 刘 涛b
于 明c 周云龙c 柴之芳a   

  1. (a中国科学院高能物理研究所核分析技术重点实验室 北京 100049)
    (b北京同步辐射装置 北京 100049)
    (c北京师范大学生命科学学院 北京 100875)
  • 投稿日期:2007-12-11 修回日期:2008-02-13 发布日期:2008-07-28
  • 通讯作者: 张智勇

Distribution in Internodal Cells of Chara and the Bonding States with the Cell Wall of Lanthanum

JIANG, Wen-Jun a LI, Zi-Jie a ZHANG, Zhi-Yong *,a ZHANG, Jing b LIU, Tao b
YU, Ming c ZHOU, Yun-Long c CHAI, Zhi-Fang a   

  1. (aKey Laboratory of Nuclear Analytical Techniques, Institute of High Energy Physics, Chinese Academy of Sciences,
    Beijing 100049)
    (b Beijing Synchrotron Radiation Facility, Beijing 100049)
    (c College of Life Sciences, Beijing Normal University, Beijing 100875)
  • Received:2007-12-11 Revised:2008-02-13 Published:2008-07-28
  • Contact: ZHANG, Zhi-Yong

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被引次数

12 | 1

利用电感耦合等离子体质谱(ICP-MS)和X射线吸收谱(XAS)研究了稀土元素镧在轮藻节间细胞中的分布和配位环境. 实验结果发现, 4 μmol•dm－3 LaCl3溶液处理12 h, 细胞中绝大部分的La存在于细胞壁且结合紧密, 各亚细胞组分中La的浓度依次为: 细胞壁>>细胞质>>液泡. XAS的结果表明La3＋在细胞壁中与9个氧原子配位, 配位环境类似于醋酸镧, 说明在细胞壁中La3＋的主要配位基团是羧基. 珊瑚轮藻具有很强的从培养介质中富集La3＋的能力, 可用于水体中稀土元素的监测与污染治理.

关键词: 镧, 稀土, 轮藻, X射线吸收谱, 电感耦合等离子体质谱

Distribution and coordination environment of lanthanum (La) in internodal cells of Chara have been determined by inductively coupled plasma-mass spectrometry and X-ray absorption spectroscopy (XAS). The results indicate that after treatment with 4 μmol•dm－3 LaCl3 solution for 12 h, most of La in cells is located in the cell wall fraction and the bonding is tight. The order of La concentrations in the subcellular fractions is cell wall>>cytoplasm>>vacuole. The results of XAS show that La3＋ is coordinated to 9 oxygen atoms in the cell wall, and the coordination environment is similar to that of lanthanum acetate, which indicates that La bonds to the cell component via carboxylate groups. According to the high ability of La accumulation, Chara can act as a biological indicator or scavenger of rare earth pollution in aqueous solutions.

Key words: lanthanum, rare earth, charophyte alga, X-ray absorption spectroscopy, inductively coupled plasma mass spectrometry