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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (15): 1786-1790. 上一篇    下一篇

研究论文

纳米ZnO修饰电极上低电位检测烟酰胺腺嘌呤二核苷酸和乙醇

白红艳 包建春 戴志晖* 刘 可

  

  1. (江苏省生物功能材料重点实验室 南京师范大学化学与环境科学学院 南京 210097)

  • 投稿日期:2008-01-21 修回日期:2008-03-21 发布日期:2008-08-14
  • 通讯作者: 戴志晖

Detection of Nicotinamide Adenine Dinucleotide (NADH) and Ethanol at ZnO Nanostructure Modified Electrode with Low Overpotential

BAI, Hong-Yan BAO, Jian-Chun DAI, Zhi-Hui* LIU, Ke   

  1. (Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Environmental Science,
    Nanjing Normal University, Nanjing 210097, China)
  • Received:2008-01-21 Revised:2008-03-21 Published:2008-08-14
  • Contact: DAI, Zhi-Hui

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1766

被引次数

39

纳米ZnO修饰玻碳电极在0.23 V对烟酰胺腺嘌呤二核苷酸(NADH)的氧化具有很好的催化活性, 与裸电极上NADH的氧化电位0.70 V相比, 该电位降低了0.47 V, 同时增强了抗干扰能力, 并在很大程度上减小了电极污染. 以乙醇脱氢酶(ADH)为例, 制备了ADH/ZnO修饰电极, 可用于脱氢酶底物乙醇的快速、灵敏检测, 并具有良好的重现性和稳定性. 研究表明纳米ZnO为构建脱氢酶底物的电化学传感器提供了一种新的生物兼容性材料.

关键词: NADH, ZnO, 电催化氧化, 传感器, 乙醇脱氢酶, 乙醇

The good catalytic activity of ZnO nanomaterial to the oxidation of nicotinamide adenine dinucleotide (NADH) was described for biosensing application. Compared to a bare electrode, the modified electrode decreased the oxidation overpotential of NADH for 0.47 V, decreased the interferences and minimized the surface fouling. With alcohol dehydrogenase (ADH) as a model, the proposed biosensor showed rapid and sensitive amperometric responses to ethanol with acceptable preparation reproducibility and stability. Nano-ZnO provided an efficient matrix for development of NADH biosensors and the prepared electrode can be used not only to detect the concentration of NADH in a biochemical reaction, but also used as the potential matrix for the construction of dehydrogenases.

Key words: NADH, ZnO, electrocatalytic oxidation, biosensor, alcohol dehydrogenase, ethanol