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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (10): 931-935.    下一篇

研究论文

DNA在氨基功能化偶氮苯自组装膜表面的固定

冯孝中，闫立军，张治红*，闫福丰，豆君，李彦山，郑先君   

  1. (河南省表界面重点实验室 郑州轻工业学院材料与化工学院 郑州 450002)
  • 投稿日期:2009-12-16 修回日期:2010-02-22 发布日期:2010-04-20
  • 通讯作者: 张治红 E-mail:mainzhh@yahoo.com.cn

DNA Immobilization onto the Self-assembled Monolayer of Azobenzene Modified by Amino Group

FENG Xiao-Zhong, YAN Li-Jun, ZHANG Chi-Gong, YAN Fu-Feng, DOU Jun, LI Pan-Shan, ZHENG Xian-Jun   

  1. (Henan Provincial Key Laboratory of Surface & Interface Science, Zheng Zhou University of Light Industry, Zhengzhou 450002)
  • Received:2009-12-16 Revised:2010-02-22 Published:2010-04-20

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被引次数

4 | 1

采用简单快速的方法制备出将DNA固定在其表面的单分子层敏感膜. 首先采用表面自组装技术将硅氧烷基偶氮苯衍生物H2NAzoCONHC3Si(OCH3)3 (APDA-N-TMSPBA)组装在硅表面, 在详细考察单分子层薄膜的化学结构、表面浸润性和分子表面形貌之后, 又通过紫外吸收光谱(UV)在位考察了硅氧烷基偶氮苯衍生物的光学异构特性. 在DNA在自组装薄膜固定后, X光电子能谱仪(XPS)结果显示出现了明显的磷元素信号, 表明DNA分子可以成功固定在自组装膜表面.

关键词: 偶氮苯, 自组装, 光学异构, DNA固定

The present work aims at how to fabricate quickly the sensitive monolayer film for DNA immobilization by the simple approach. Firstly, 4-[(4-aminophenyl)diazenyl]-N-[3-(trimethoxysilyl)propyl] benzamide (APDA-N-TMSPBA) was self-assembled onto the silicon surface to form a monolayer film. After the detailed investigation of the chemical structure, surface wettability and surface morphology of the self-assembled monolayer (SAM), the reversible photoisomerization of (APDA-N-TMSPBA) was characterized in situ by UV-Vis absorption spectrum. Moreover, after the DNA immobilization onto SAM surface, the obvious signal of P element was observed. It demonstrates that the DNA molecules could be anchored onto the SAM successfully.

Key words: azobenzene, self-assembly, photoisomerization, DNA immobilized