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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (08): 955-959. 上一篇    下一篇

研究论文

糖芯片技术研究琼胶寡糖与凝集素RCA120相互作用

王玉峰1，于广利*,1,2，韩章润2，吴建东2，赵小亮2，柴文刚2,3   

  1. (1海洋药物教育部重点实验室 中国海洋大学 青岛 266003)
    (2山东省糖科学与糖工程重点实验室 青岛 266003)
    (3 Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Harrow, Middlesex HA1 3UJ, United Kingdom)
  • 投稿日期:2010-10-20 修回日期:2010-11-25 发布日期:2010-12-13
  • 通讯作者: 王玉峰 E-mail:wy511813@gmail.com
  • 基金资助:

    国际科技合作专项;国家海洋局公益性行业专项;国家自然科学基金;长江学者和创新团队发展计划

Interaction of Agarose-based Oligosaccharides with Agglutinin RCA120 Using Glycochip Technology

Wang Yufeng1 Yu Guangli*,1,2 Han Zhangrun2 Wu Jiandong2 Zhao Xiaoliang2 Chai Wengang2,3   

  1. (1 Key Laboratory of Marine Drugs, Ministry of Education, Ocean University of China, Qingdao 266003)
    (2 Shandong Provincial Key Laboratory of Glycoscience and Glycotechnology, Qingdao 266003)
    (3Glycosciences Laboratory, Faculty of Medicine, Imperial College London, Harrow, Middlesex HA1 3UJ, United Kingdom)
  • Received:2010-10-20 Revised:2010-11-25 Published:2010-12-13
  • Contact: Yufeng Wang E-mail:wy511813@gmail.com

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以琼胶糖为原料, 通过酸法与酶法降解以及凝胶过滤色谱分离, 获得了9种寡糖. 采用还原胺化法将其与1,2- 二-十六烷基-3-磷脂酰乙醇胺甘油(DHPE)偶联, 获得了相应拟糖脂. 采用糖芯片技术, 通过与标准寡糖(LNnT与LNT)比较, 评价了其与凝集素RCA120的相互作用. 首次发现, 酸法水解得到的琼胶寡糖与RCA120作用力明显高于LNnT, 表明具有Galβ1,4AnG-R结构类型的寡糖与RCA120亲和力强于Galβ1, 4GlcNAc-R, 且亲和力的大小与聚合度无关.

关键词: 琼胶寡糖, 拟糖脂, 糖芯片, RCA120, 相互作用

Nine oligosaccharides were obtained from agarose by chemical and enzymatic degradation and gel filtration chromatography. All oligosaccharides were linked to 1,2-dihexadecyl-sn-glycero-3-phospho- ethanolamine (DHPE) by a reductive animation reaction to acquire neoglycolipids. Compared with standard oligosaccharides (LNnT and LNT), the affinity of agaro-oligosaccharides to RCA120 was studied using glycochip technique. We firstly found the higher affinity of oligosaccharides with structure of Galβ1,4AnG-R than that of Galβ1,4GlcNAc-R to RCA120 compared with LNnT, and the affinity have no relation with degree of polymerization.

Key words: agaro-oligosaccharide, neoglycolipid, glycochip, RCA120, interaction