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化学学报
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化学学报 ›› 2002, Vol. 60 ›› Issue (8): 1459-1464. 上一篇    下一篇

研究论文

PVP/Pd/IrO_2/Nafion修饰微电极用于成纤维细胞中一氧化氮释放的研究

鲜跃仲;徐继明;陆嘉星;刘梅川;蔡琪;金利通   

  1. 华东师范大学化学系.上海(200062)
  • 发布日期:2002-08-15

Direct Monitoring of Nitric Oxide Release from Fibrocytes with PVP/Pd/IrO_2/Nation Chemically Modified Microelectrode

Xian Yuezhong;Xu Jiming;Lu Jiaxing;Liu Meichuan;Cai Qi;Jin Litong   

  1. Department of Chemistry, East China Normal University.Shanghai (200062)
  • Published:2002-08-15

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被引次数

1

采用PVP/Pd/IrO_2/Nafion修饰电极对成纤维细胞中NO的释放情况进行了研究 。结果表明，在正常状态下，采用NO前体L-精氨酸和乙酰胆碱对成纤维细胞进行刺 激后没有NO的释放；当用脂多糖进行诱导后，则释放出高浓度的NO，加入L-精氨酸 和乙酰胆碱都促进了NO的合成，而L-NNA的加入则逆转了L-精氨酸和乙酰胆碱的作 用。

关键词: 钯, 氧化铱, 化学修饰电极, 一氧化氮, 精氨酸, 乙酰胆碱

PVP/Pd/IrO_2/Nafion microsensor was fabricated to measure nitric oxide production in fibrocytes directly. In the experiments, fibrocytes did not release nitric oxide after stimulated with L- arginine and acetylcholine under normal condition. After induced with lipopolysaccharide, NO was produced in high concentration and NO levels were increased after stimulated with L-arginine and acetylcholine. On the other hand, L-N~w-nitro-arginine, an NO synthase inhibitor, decreased the action of L-arginine and acetylcholine.

Key words: PALLADIUM, IRIDIUM OXIDE, CHEMICAL MODIFIED ELECTRODE, NITROGEN MONOXIDE, ARGININE, ACETYLCHOLINE

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