关键词: 紫外分光光度法, 蛋白质, 相互作用, 酸性铬蓝K, 血清白蛋白

In this paper, the study on the reaction between Acidic Chrome Blue K (ACBK) and Bovine Serum Albumin (BSA) was reported. The binding numbers and the binding constants were given at different pH by using three methods with relevent comparison. It was proved that there form in the presence of BSA.

Key words: ULTRAVIOLET SPECTROPHOTOMETRY, PROTEIN, INTERACTIONS, SERUM ALBUMIN