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化学学报
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化学学报 ›› 2009, Vol. 67 ›› Issue (9): 957-963. 上一篇    下一篇

研究论文

纳米TiO2-Cu2O复合材料可见光催化杀伤人宫颈肿瘤细胞研究

徐 芬a 查玉平b 王国秀b 王 艳a 李家麟*,a

  

  1. (a华中师范大学纳米科技研究院 武汉 430079)
    (b华中师范大学生命科学学院 武汉 430079)

  • 投稿日期:2008-05-07 修回日期:2008-07-23 发布日期:2009-05-14

Damaging Effect of Visible Light-assisted Illuminated TiO2-Cu2O Nano-composite on Hela Cell

Xu, Fen a Zha, Yuping b Wang, Guoxiu b Wang, Yan a Li, Jialin *,a   

  1. (a Institute of Nanoscience and Nanotechnology, Huazhong Normal University, Wuhan 430079)
    (b College of Life Science, Huazhong Normal University, Wuhan 430079)
  • Received:2008-05-07 Revised:2008-07-23 Published:2009-05-14

PDF

1496

被引次数

18

以人宫颈肿瘤细胞(Hela细胞)为研究对象, 研究了可见光催化(光强为50 mW/cm2)条件下, 该复合材料Fenton作用对细胞的凋亡诱导作用和细胞周期的影响, 并对抗肿瘤作用机理进行探讨. 结果表明, 该复合材料对肿瘤细胞具有明显的杀伤作用, 抑制Hela细胞增殖, 降低细胞存活率, 诱导Hela细胞产生细胞凋亡. 此外, 还能够引起细胞周期各时相改变, 使细胞生长阻滞于G2/M期. 并引发细胞氧化应激反应的发生, 最终破坏胞内抗氧化酶体系的平衡. 由此可见, 纳米TiO2-Cu2O复合材料在抗肿瘤的可见光疗应用中具有一定的应用价值.

关键词: 纳米TiO2-Cu2O复合材料, Hela细胞, 可见光催化, 抗肿瘤

TiO2-Cu2O nano-composite was obtained by a novel electrochemical method. The effects of TiO2-Cu2O Fenton coating on proliferation and induced apoptosis in Hela cells were investigated. The changes of cell cycle were observed too. Results revealed that visible light-photocatalysis (50 mW/cm2) under 3 min resulted in considerably low cell viability and produced characteristic apoptotic events in Hela cells including morphological changes, DNA ladder formation and increased number of cells with G2/M phase. Furthermore, the mRNA level of IκBα was significantly increased under phototreatment for 5 min on Hela cells. The antioxidant defense system and MDA were investigated. It was found that the imbalance of the antioxidant enzyme system induced under the phototreatment contributed to oxidative stress. These results indicated that the TiO2-Cu2O Fenton coating led to enhanced growth inhibition and apoptotic production. The TiO2-Cu2O nano-composites might be used as a new anti-cancer modality to cancer treatment under a visible light condition.

Key words: TiO2-Cu2O nano-composite, Hela cell, visible light-photocatalysis, anti-cancer