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化学学报
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化学学报 ›› 1993, Vol. 51 ›› Issue (7): 683-689. 上一篇    下一篇

研究论文

生物分子电催化的研究2:血红蛋白在甲酚固紫修饰石黑电极上的电催化

韩吉林;陈洪渊;高鸿   

  1. 南京大学化学系
  • 发布日期:1993-07-15

Electrocatalysis of biomolecules at modified electrodes pt.2:catalytic reduction of hemoglobin at chemically modified electrode containing cresyl east violet

HAN JILIN;CHEN HONGYUAN;GAO HONG   

  • Published:1993-07-15

PDF

563

被引次数

25

本文将甲酚固紫吸附在石墨电极上以制成修饰电极,它是一种能够加快血红蛋白在电极上电子传递速度的新的电子传递媒介体.在+09.2V～-0.4V(VS.SCE)电位范围内,吸附态的甲酚固紫表现出相当可逆的两电子氧化还原行为,同时有两个质子参加.在pH5.5NaOAc-HOAc缓冲溶液中,其表面式量电位E^0'为-0.18V,表观电子传递速率K~S为0.71s^-1.甲酚固紫修饰电极具有良好的稳定性,它能够大大加快血红蛋白在电极上的电子传递速度,使其具有很好的电流响应,可应用于血红蛋白的测定.

关键词: 血红蛋白, 化学修饰电极, 电催化, 石墨电极

The chem. modified electrode (CME), which was constructed by adsorbing a new mediator cresyl fast violet onto the spectrog. graphite electrode, shows electrocatalytic activity to the reduction of Hb. In the potential range +0.2V~-0.4V the mediator cresyl fast violet behaves as two electrons reversible redox process involving two protons. In the medium of 0.2 mol譫m -3NaOAc-HOAc (pH=5.5) the surface formal potential E0' and apparent electron-transfer rate of the adsorbed mediator cresyl fast violet are -0.18V (vs. SCE) and 0.71s-1, resp. The CME shows a good stability, and a good current response of Hb. It can be used as a sensor for determing Hb in FIA of LC-EC method.

Key words: HEMOGLOBIN, CHEMICAL MODIFIED ELECTRODE, ELECTRO-CATALYSIS, GRAPHITE ELECTRODE

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