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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (1): 91-96. 上一篇    下一篇

研究论文

均苯三酰胺桥联三环糊精/Zn2+/金刚烷衍生物三元超分子体系的合成及其羧肽酶A模拟研究

陈湧，迟恒，刘育*   

  1. (南开大学化学系 元素有机化学国家重点实验室 天津 300071)
  • 投稿日期:2007-06-14 修回日期:2007-07-31 发布日期:2008-01-14
  • 通讯作者: 刘育

Synthesis and Carboxypeptidase A Mimic of Supramolecular Triads Formed by Benzene-1,3,5-tricarboxylamido-bridged Tri(b-Cyclodextrin), Zn2+ and Admantane Derivatives

CHEN Yong; CHI Heng; LIU Yu*   

  1. (Department of Chemistry, State Key Laboratory of Elemento-Organic Chemistry, Nankai University, Tianjin 300071)
  • Received:2007-06-14 Revised:2007-07-31 Published:2008-01-14

PDF

853

被引次数

20

本文合成了一种均苯三酰胺基桥联三(b-环糊精) 1及其锌(II)配合物 2, 进而通过与金刚烷衍生物的包结配位作用制备了三种均苯三酰胺桥联三环糊精/Zn2+/金刚烷衍生物4-6. 利用茚三酮显色实验和圆二色谱研究了其作为羧肽酶A模拟物对牛血清白蛋白水解反应的酶促性能和不同pH下的变构能力, 推测了可能的酶促反应机理. 结果表明1/Zn2+/金刚烷-1-乙酸三元超分子体系在中性条件显示出高达10.43 U·mg-1的酶活力

关键词: 桥联三环糊精, 金刚烷衍生物, 三元超分子体系, 羧肽酶A, 模拟酶

A benzene-1,3,5-tricarboxylamido-bridged tri(b-cyclodextrin) and its Zn2+ complex 2 are synthesized. Inclusion complexations of 2 with admantane derivatives give three supramolecular triads formed by tri(b-cyclodextrin), Zn2+ and admantane derivatives. Their carboxypeptidase activities and conformational mutating abilities under different pH were explored by ninhydrin colorimetric and circular dichroism spectrometric studies, and the possible mechanism was discussed. The results show that the 1/Zn2+/1-adamantaneacetic acid system gave the highest enzymatic activity up to 10.43 U·mg-1at a neutral condition.

Key words: bridged tri(b-cyclodextrin), admantane derivatives, supramolecular triad, carboxypeptidase A, enzyme mimic