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化学学报
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化学学报 ›› 2008, Vol. 66 ›› Issue (4): 424-432. 上一篇    下一篇

研究论文

DNA在碳纳米管表面的固定、表征及损伤的电化学检测

刘姝娜, 吴萍, 周泊, 周耀明, 蔡称心*   

  1. (南京师范大学化学与环境科学学院 生物功能材料江苏省重点实验室
    分子医学生物技术江苏省重点实验室 南京 210097)
  • 投稿日期:2007-07-11 修回日期:2007-09-21 发布日期:2008-02-28
  • 通讯作者: 蔡称心

Immobilization, Characterization of DNA on the Surface of Carbon Nanotube and Electrochemical Detection of DNA Damage

LIU Shu-Na; WU Ping; ZHOU Bo; ZHOU Yao-Ming; CAI Chen-Xin*   

  1. (College of Chemistry and Environmental Science, Jiangsu Key Laboratory for Biofunctional Materials, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, Nanjing 210097)
  • Received:2007-07-11 Revised:2007-09-21 Published:2008-02-28
  • Contact: CAI Chen-Xin

PDF

697

被引次数

26 | 3

将DNA通过PDDA [poly(dimethyldiallylammonium chloride)]固定在单壁碳纳米管(CNT)表面, 形成DNA-PDDA-CNT纳米复合体, 用AFM、UV-Vis、Raman光谱及交流阻抗对其进行了表征. 用伏安法研究了1-苯基偶氮-2-萘酚(PN)与固定在CNT表面的DNA的相互作用, 结果表明PN与DNA的作用方式为嵌入作用；并且, PN能用作电化学检测DNA化学损伤的探针分子. 本文电化学检测DNA损伤的优点在于PN的氧化还原式量电位E0'≈0.1 V (vs. SCE, pH 5.5), 能有效降低其它电活性物质对DNA损伤电化学检测的干扰.

关键词: 碳纳米管, DNA, DNA损伤, 电化学检测

DNA-PDDA-CNT nanocomposites were fabricated by immobilizing DNA on the surface of single-walled carbon nanotubes (CNT) via poly(dimethyldiallylammonium chloride) (PDDA). The atomic force microscopy (AFM), ultraviolet-visible (UV-Vis), Raman spectroscopy and impedance spectroscopy (EIS) were used to characterize the growth processes of the nanocomposites. The cyclic voltammetric results indicate that the interaction between 1-phenylazo-2-naphthol (PN) and the immobilized DNA is intercalative binding, and PN can be used as a probe for detection of DNA damage. PN seems to be an attractive probe for DNA damage analysis since its redox peak is at ca. 0.1 V (vs. SCE, pH 5.5), where interference should be minimal.

Key words: carbon nanotube, DNA, DNA damage, electrochemical detection