关键词: 二氧六环 P, 酶电极, 亚铁氰化物, 辣根过氧化物酶, 生物电催化, 电子传递体

The horseradish peroxidase (HRP) electrode was made by crosslinkage method, and its electrochemical behaviour was studied in 1,4-dioxane media. It is shown that the immobilized HRP can maintain its activity in organic phase and the direct electron transfer occurs between HRP and the electrode substrate, hence the HRP electrode can catalyze the electrochemical reduction of H~2O~2 in the absence of mediators. When HRP and K~4Fe(CN)~6 are comodified on the basis electrode, the performance of HRP electrode will be improved significantly since K~4Fe(CN)~6 acts as the electron transfer mediator in the redox process of enzyme. According to the kinetic parameters obtained in different conditions, the factors influencing the performance of the HRP electrode are discussed.

Key words: DIOXANE P, ENZYME ELECTRODES, FERROCYANIDE