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化学学报
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化学学报 ›› 2006, Vol. 64 ›› Issue (24): 2396-2402. 上一篇    下一篇

研究论文

铁氧化还原蛋白在多壁碳纳米管上的固定、表征及直接电子转移

吕亚芬, 蔡称心*   

  1. (南京师范大学化学与环境科学学院 分子医学生物技术江苏省重点实验室 南京 210097)
  • 投稿日期:2006-06-22 修回日期:2006-07-22 发布日期:2006-12-28
  • 通讯作者: 蔡称心

Immobilization, Characterization and Direct Electron Transfer Reac-tion of Ferredoxin on Multi-walled Carbon Nanotube

LÜ Ya-Fen; CAI Chen-Xin*   

  1. (College of Chemistry and Environmental Science, Jiangsu Key Laboratory for Molecular and Medical Biotechnology, Nanjing Normal University, Nanjing 210097)
  • Received:2006-06-22 Revised:2006-07-22 Published:2006-12-28
  • Contact: CAI Chen-Xin

PDF

994

被引次数

49 | 7

将来源于Spinacia Oleracea的铁氧化还原蛋白(ferredoxin, SOFd)固定在多壁碳纳米管(CNT)表面, 紫外-可见及红外光谱表明, SOFd在CNT表面没有变性, 仍保持原来的二级空间结构. 循环伏安结果表明, SOFd在CNT表面能进行有效和稳定的直接电子转移反应, 伏安曲线上出现一对良好的、几乎对称的氧化还原峰, 式量电位E0'为(－570.4±1.5) mV (vs. SCE, 0.1 mol/L磷酸盐缓冲液), 且不随扫速和溶液pH值的变化而变化. SOFd直接电子转移的表观速率常数ks为(0.73±0.04) s－1.

关键词: 碳纳米管, 化学修饰电极, 铁氧化还原蛋白, 直接电化学

Spinacia Oleracea ferredoxin (SOFd) was immobilized on the surface of the multi-walled carbon nanotubes (CNT). The results of UV-Vis spectra and Fourier transform infrared (FT IR) spectra indicated that SOFd retained the essential feature of its secondary structure on the surface of CNT. The cyclic voltammetric results showed that the SOFd on the surface of CNT could undergo effective and stable direct transfer-electron reaction. The cyclic voltammogram was characterized by a well-defined and nearly sym-metrical redox peaks with formal redox potential (E0') of (－570.4±1.5) mV (vs. SCE, in 0.1 mol/L PBS), which was almost independent on the scan rates and solution pH. The apparent heterogeneous electron transfer rate constant (ks) for the direct electron-transfer of SOFd was estimated to be (0.73±0.04) s－1.

Key words: carbon nanotube, chemically modified electrode, ferredoxin, direct electron transfer