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化学学报
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化学学报 ›› 2001, Vol. 59 ›› Issue (5): 659-664. 上一篇    下一篇

研究论文

β-lactoglobulin 与磷脂复合膜形成过程中相互 作用的动态研究

张翼;颜玲珑;毕只初;李峻柏;董栋;刘忠范   

  1. 中国科学院化学研究分子科学中心;北京大学化学系.北京(100871)
  • 发布日期:2001-05-15

Dynamic study of the interaction between β-lactoglobulin and phospholipids during complex film formation

Zhang Yi;Yan Linglong;Bi Zhichu;Li Junbai;Dong Dong;Liu Zhongfan   

  1. Beijing Univ., Dept. of Chem.Beijing(100871)
  • Published:2001-05-15

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利用ADSA系统分别研究了蛋白质β-lactoglobulin在不同pH下与三种磷脂DPPC(中性头部基因)、DPPE(部分正电头部基团)、DPPA(部分带负电头部基因)的吸附动力学，结合AFM技术，讨论在弯曲的液/液界面上蛋白质与磷脂之间各种相互作用对复合膜生成的影响。认为这些相互作用的影响是动态变化着的；在吸附反应的不同阶段各类作用分别成为主导因系。

关键词: 磷脂, 蛋白膜, 复合薄膜, 相互作用

Dynamic interfacial tension of phospholipid /protem adsorption was studied by ADSA( axisymmetric drop shape analysis, DASA) to gain the information about the protein /phosphoilpid multilayer. AFM was used to study the morpholoigy of the ciomplex film. DPPE ( dipalmitoyl phosphatidylethanolamine ), DPPC( dipalnitoyl phosphatidylcholin), DPPA (dipalnitoyl phosphatidic acid) exhibitied quite different adsorption behavior in the step due to the net charge of their head group, but the interaction between phospholipid and protein seemed beyond the statistic force during the construction of protein /lipid multilayers when the different adsorption curves tended to coincide at the end. The adsorption process was dominated by different kind interaction at the different steps respectively.

Key words: PHOSPHOLIPID, LAMINATED FILMS, INTERACTIONS

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