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化学学报
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化学学报 ›› 2001, Vol. 59 ›› Issue (10): 1798-1802. 上一篇    下一篇

研究论文

特异性吗啡抗原和单克隆抗体的制备

王能东;陈家华;张秀;陈柳民;金声   

  1. 北京大学化学学院;北京大学生物有机与分子工程教育部重点实验室
  • 发布日期:2001-10-15

Production of specific morphine antigen and the monoclonal antibodies

Wang Nengdong;Chen Jiahua;Zhang Xiu;Chen Liumin;Jin Sheng   

  • Published:2001-10-15

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930

被引次数

2

吗啡分子的不同部位与蛋白偶联诱导出的抗体的专一性差异很大。为减少交叉反应，提高抗体分子对游离吗啡的特异性识别，选择吗啡分子N位进行修饰，合成了半抗原降吗啡，并设计和合成不同的连接臂，将半抗原用不同的连接臂与不同的载体蛋白共价结合分别制备了免疫抗原和筛选抗原，经细胞融合和筛选，成功地获得了5株可分汔抗吗啡单克隆抗体的细胞株：28H10,29D5,36G3,42D5,43C4。

关键词: 吗啡, 单克隆抗体, 吗啡半抗原, 蛋白, 偶联

Those antibodies elicited by different tether site for attachment to carrier protein differ geratly in specificity. In order to reduce cross reactivity and enhance the specific recognition between the antibody and free morphine, the hapten was synthesized by the modification in N-site of morphine. The conjuagtes of hapten and proteins, used to immunize and screen, were prepared respectively by using different linkers to couple to different carrier proteins, and the 5 murine monoclonal antibodies against morphine, 28H10, 29D5,36G3, 42D5 and 43C4 were produced.

Key words: MORPHINUM, MONOCLONAL ANTIBODY, PROTEIN, COUPLED

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