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化学学报
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化学学报 ›› 2010, Vol. 68 ›› Issue (14): 1415-1420. 上一篇    下一篇

研究论文

用二维核磁共振研究[Co(phen)2(HNAIP)]Cl3配合物与错配及正常寡聚核苷酸的作用

席小莉1，杨曼曼2，陈绘丽1，杨频*,1   

  1. (1山西大学分子科学研究所化学生物学与分子工程教育部重点实验室 太原 030006)
    (2山西大学化学化工学院 太原 030006)
  • 投稿日期:2009-12-28 修回日期:2010-01-26 发布日期:2010-03-11
  • 通讯作者: 杨频 E-mail:yangpin@sxu.edu.cn

Study on the Interaction between Mismatched Oligonucleotide and Normal Oligonucleotide with [Co(phen)2(HNAIP)]Cl3 by 2D NMR

Xi Xiaoli1 Yang Manman2 Chen Huili1 Yang Pin*,1   

  1. (1 Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education of China at College of Chemistry and Chemical Engineering, Institute of Molecular Science, Taiyuan 030006)
    (2 The College of Chemistry, Shanxi University, Taiyuan 030006)
  • Received:2009-12-28 Revised:2010-01-26 Published:2010-03-11

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为了开发能识别正常与错配核酸的探针, 首次合成了[Co(phen)2(HNAIP)]Cl3金属配合物, 并利用二维核磁研究了此配合物与错配d(CCGAATGAGG)2及正常d(CCTAATTAGG)2寡聚核苷酸的相互作用, 首次发现, [Co(phen)2(HNAIP)]Cl3是从“大沟”插入到错配碱基对G3A4和G7A8之间, 能够很好地识别G:A错配; 而[Co(phen)2(HNAIP)]Cl3与正常的寡聚核苷酸的作用, 则是从小沟插入, 即[Co(phen)2(HNAIP)]Cl3与错配和正常寡聚核苷酸在不同位置及不同沟槽存在明显的插入式结合、并且有明显的差异, 这就有可能被用来作为识别正常与错配寡聚核苷酸的探针.

关键词: 二维NOSY谱, 寡聚核苷酸, DNA

In order to develop the probe that can identify mismatched oligonucleotide and normal oligonucleotide, we synthesized [Co(phen)2(HNAIP)]Cl3 metal complex firstly, and studied the interaction between mismatched oligonucleotide d(CCGAATGAGG)2 and normal oligonucleotide d(CCTAATTAGG)2 with [Co(phen)2(HNAIP)]Cl3 by 2D NMR. The results indicate that the complex binds the mismatched oligonucleotide by intercalation with the HNAIP ligand selectively inserted from the major groove between the stacked bases in the G3A4 and G7A8 region. So, it can identify G:A mismatch. At the same time, the complex binds the normal oligonucleotide by intercalation with the HNAIP ligand from the minor groove between the stacked bases in the terminal region. Because [Co(phen)2(HNAIP)]Cl3 can interact with mismatched oligonucleotide and normal oligonucleotide on different location and by different style, it can be used as a probe to identify mismatched oligonucleotide and normal oligonucleotide.

Key words: 2D NMR, oligonucleotide, DNA