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化学学报
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化学学报 ›› 2011, Vol. 69 ›› Issue (10): 1191-1196. 上一篇    下一篇

研究论文

烟嘧磺隆分子印迹聚合物表面等离子共振传感器的制备与分析应用

赵楠1, 陈长宝1, 周杰*,1, 范加金2   

  1. (1山东农业大学化学与材料科学学院 泰安 271018)
    (2山东泰邦生物制品有限公司 泰安 271000)
  • 投稿日期:2010-11-16 修回日期:2011-01-05 发布日期:2011-01-26
  • 通讯作者: 周杰 E-mail:zhoujie@sdau.edu.cn
  • 基金资助:

    国家863 计划专题课题基金

Preparation of An SPR Sensor Based on Nicosulfuron Molecularly Imprinted Polymer and Its Analytical Application

Zhao Nan1|Chen Changbao1|Zhou Jie*,1|Fan Jiajin2   

  1. (1 Shandong Agricultural University, Taian, Shandong 271018)
    (2 Shandong Taibang Biological Products Limited Company, Taian, Shandong 271000)
  • Received:2010-11-16 Revised:2011-01-05 Published:2011-01-26

PDF

1590

被引次数

19

利用分子印迹技术与表面等离子共振光谱联用, 建立了对磺酰脲类除草剂烟嘧磺隆的检测方法. 实验过程中, 探讨了pH值对分子印迹膜吸附目标化合物特性的影响, 并在最佳pH下对其吸附和选择性能进行了评价. 与非印迹聚合物相比, 烟嘧磺隆印迹聚合物对烟嘧磺隆吸附效率比烟嘧磺隆类似物高. 该方法的线性范围为5.0×10－12～25× 10－12 mol/L, 烟嘧磺隆和分子印迹聚合物之间的结合常数为1.6×1010 L/mol, 吉布斯自由能变化值为－58.148 kJ/mol. 研究结果表明该方法具有简单、快速、灵敏度高、重复性好等特点, 适用于自来水和土壤中磺酰脲类除草剂烟嘧磺隆的测定, 基于信噪比为3时, 自来水和土壤的检出限分别为5.62×10－14和1.01×10－13 mol/L, 平均回收率分别为85.6%和76.6%, 相对标准偏差分别为1.78%和3.21%.

关键词: 分子印迹聚合物, 表面等离子共振传感器, 烟嘧磺隆

A new SPR sensor for the detection of nicosulfuron herbicide was prepared based on a combination of molecularly imprinted technique with surface plasmon resonance (SPR) spectroscopy. In the process of this experiment, the effect of pH value on the adsorption of the imprinted membrane to the analyte was investigated. Meanwhile, we also evaluated the properties of its adsorptivity and selectivity at the optimal pH. Compared to the non-imprinted polymer, the imprinted polymer showed higher adsorption efficiency for nicosulfuron than that of other structurally related analogues. The method showed good linearity between 5.0×10－12～25×10－12 mol/L. The association constant of the imprinted sites in the composite to nicosulfuron (Kass) was calculated to be 1.6×1010 L/mol and the change in Gibb s free energy was －58.148 kJ/mol. The results indicated that the developed SPR sensor for the detection of nicosulfuron possessed the advantages such as simplicity, high-speed, high sensitivity and good recovery and when it was used to determine the nicosulfuron herbicide in the tap water and soil, based on a signal to noise ratio of 3, the detection limits were 5.62×10－14 mol/L and 1.01×10－13 mol/L, the mean recoveries were 85.6% and 76.6%, the relative standard deviations (RSD) obtained were 1.78% and 3.21% for the tap water and soil, respectively.

Key words: molecularly imprinted technique, surface plasmon resonance sensor, nicosulfuron

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