关键词: 双功能单体, 分子印迹, 葡萄糖, 类特异性, 葡萄糖苷, 超顺磁性纳米球

In this paper, dual functional monomers (concanavalin A and aminobenzeneboronic acid) method is applied to molecular imprinting technique, three synthetic methods of functional monomer were designed and their ability to adsorb glucose was evaluated. Step-by-step method (A1) was found to be a good synthetic method for selective adsorption, which could adsorb glucose powerfully. A kind of magnetic molecularly imprinted polymers (MMIPs) was synthesized by step-by-step method, in which superparamagnetic Fe₃O₄ nanoparticles were firstly synthesized by hydrothermal synthetic method and uniform SiO₂-coated Fe₃O₄ (Fe₃O₄@SiO₂) nanoparticles were prepared via Sol-gel method. The following Fe₃O₄@SiO₂ reacted with (3-aminopropyl)triethoxysilane to produce Fe₃O₄@SiO₂@NH₂. The aminated nanoparticles combine with concanavalin A to form Fe₃O₄@SiO₂@NH₂@ConA in the presence of disuccinimidyl suberate. Subsequently, the single-functionalized nanoparticles binded with aminobenzeneboronic acid to form Fe₃O₄@SiO₂@NH₂@APBA-ConA in the presence of disuccinimidyl suberate. Eventually, the MMIPs for glucose selective recognition, with core-shell structure, were synthesized by surface molecular imprinting method, using glucose (Glu) as template molecule, concanavalin A (ConA) and aminobenzeneboronic acid (APBA) as dual functional monomers, N,N'-methylenebisacrylamide as the crosslinking agent, ammonium peroxydisulfate as the initiator, superparamagnetic nanoparticles as magnetic carrier. The MMIPs were characterized by X-ray diffraction (XRD), energy dispersive spectrometer (EDS), scanning electron microscopy (SEM), Fourier transform infrared spectra (FT-IR), vibrating sample magnetometer (VSM) and thermogravimetric analysis (TGA). The SEM showed the nanoparticles were highly dispersive and uniform. The EDS, FT-IR and TGA revealed the success of step-by-step method. The VSM demonstrated the saturation magnetization values of Fe₃O₄ and MMIPs were 96.661 emu/g and 45.064 emu/g, respectively. The study on kinetics of MMIPs showed that the adsorption reaction could be considered as a second order reaction. The thermodynamics research revealed that the adsorption of MMIPs was fitted to Langmuir isotherms. The study on selective adsorption displayed that MMIPs had maximum imprinting factor of 2.93 and best selectivity. The class specific study of MMIPs showed that it could be applied to extraction and separation of glucosides.

Key words: dual functional monomers, molecular imprinted, glucose, class specific, glucoside, superparamagnetic nanoparticles