基于铜-巯基配位聚合物电化学催化的新型乙酰胆碱酯酶电化学传感器

doi:10.6023/A17070321

化学学报 ›› 2017, Vol. 75 ›› Issue (11): 1109-1114.DOI: 10.6023/A17070321 上一篇下一篇

所属专题：纳米传感分析

研究论文

基于铜-巯基配位聚合物电化学催化的新型乙酰胆碱酯酶电化学传感器

王琴^a, 聂舟^a, 胡宇芳^a,b, 姚守拙^a

a 湖南大学化学化工学院化学生物传感与计量学国家重点实验室长沙 410082;
b 宁波大学材料科学与化学工程学院宁波 315211

投稿日期:2017-07-18 发布日期:2017-09-04
通讯作者: 聂舟, 胡宇芳 E-mail:niezhou.hnu@gmail.com;huyufang@nbu.edu.cn
基金资助:
项目受国家自然科学基金（Nos.21575038，21235002，21605089，21305037）、国家自然科学创新性群体基金（No.21521063）和湖南省自然科学基金（No.2015JJ1005）资助.

Electrochemical Assay for Acetylcholinesterase Activity Detection Based on Unique Electro-catalytic Activity of Cu(II)-thiol Coordination Polymer

Wang Qin^a, Nie Zhou^a, Hu Yufang^a,b, Yao Shouzhuo^a

a College of Chemistry and Chemical Engineering, State Key Laboratory of Chemo/Biosensing and Chemometrics, Hunan University, Changsha 410082;
b Faculty of Materials Science and Chemical Engineering, Ningbo University, Ningbo 315211

Received:2017-07-18 Published:2017-09-04
Contact: 10.6023/A17070321 E-mail:niezhou.hnu@gmail.com;huyufang@nbu.edu.cn
Supported by:
Project supported by the National Natural Science Foundation of China (Nos. 21575038, 21235002, 21605089, and 21305037), the Foundation for Innovative Research Groups of NSFC (No. 21521063), and the Natural Science Foundation of Hunan Province (No. 2015JJ1005).

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摘要/Abstract

乙酰胆碱酯酶（AChE）是普遍存在于周围神经系统中的一种关键酶，可特异性催化底物乙酰胆碱发生水解反应，产生胆碱和醋酸盐，这一过程与阿尔兹海默症和炎症过程等相关疾病有着密切的关系.在本研究中通过硫代胆碱（TCh）与Cu（Ⅱ）反应合成了一种铜-巯基配位聚合物[命名为TCh-Cu（Ⅱ）CP]，并发现该聚合物具有独特的电催化活性，基于此构建了一种用于AChE活性检测和抑制剂筛选的新型电化学生物传感平台.结果表明，该聚合物修饰在电极表面后可以催化邻苯二胺产生明显的电化学信号，信号强度与AChE浓度在0.05至100 mU·mL^-1范围内具有良好的线性关系，检出限为0.03 mU·mL^-1（S/N=3），通过该方法还实现了AChE抑制剂的筛选.和传统的检测方法相比，该传感方法具有制备简单、选择性高和灵敏度高等特点.

关键词: 电化学传感, TCh-Cu(II)配位聚合物, 乙酰胆碱酯酶, 抑制剂筛选

Acetylcholinesterase (AChE), as a key enzyme, ubiquitously exists in the peripheral nervous system. It mainly functions terminating neurotransmission at the cholinergic synapse through the rapid hydrolysis of acetylcholine (a neurotransmitter) into choline and acetate, which is intimately related to Alzheimer's disease, inflammatory processes, and nerve-agent poisoning. In this study, we developed a novel electrochemical method for probing AChE activity and screening its potential inhibitor based on the in-situ formation of thiocholine-Cu(Ⅱ) coordination polymer[denoted as TCh-Cu(Ⅱ) CP]. The detection mechanism is on the basis of the concept, that is, AChE could catalyze the hydrolysis of its substrate acetylthiocholine (ATCh) to produce a thiol-compound thiocholine (TCh). Subsequently, TCh reacted with Cu(Ⅱ) to form a positively charged TCh-Cu(Ⅱ) CP via S-Cu bond. Since the graphene (GO) is a negative compound due to its plenty of carboxyl groups, TCh-Cu(Ⅱ) CP could be adsorbed onto the graphene-modified glassy carbon electrode (GO/GCE) via electrostatic interaction. What's more, the CP/GO/GCE can electro-catalyze the O-phenylenediamine (OPD), generating a high current signal. We also conducted a series of experiments to verify the formation and electro-catalysis of TCh-Cu(Ⅱ) CP and investigated the selectivity of the TCh-Cu(Ⅱ) CP-based assay for AChE. As a result, it is clearly observed that the electrochemical response gradually increases with the increasing concentrations of AChE (0.05~100 mU·mL^-1) and a detection limit of our method is estimated to be ca. 0.03 mU·mL^-1 (S/N=3). Furthermore, compared to the traditional methods, our electrochemical assay has more simple detection procedure with better sensitivity and selectivity and has great potential in applications in many important areas, such as neurobiology, toxicology, and pharmacology, especially for the effective treatment of Alzheimer's disease.

Key words: electrochemical biosensor, TCh-Cu (II) coordination polymer, acetylcholinesterase, inhibitor screening

引用此文

王琴, 聂舟, 胡宇芳, 姚守拙. 基于铜-巯基配位聚合物电化学催化的新型乙酰胆碱酯酶电化学传感器[J]. 化学学报, 2017, 75(11): 1109-1114.

Wang Qin, Nie Zhou, Hu Yufang, Yao Shouzhuo. Electrochemical Assay for Acetylcholinesterase Activity Detection Based on Unique Electro-catalytic Activity of Cu(II)-thiol Coordination Polymer[J]. Acta Chim. Sinica, 2017, 75(11): 1109-1114.

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基于铜-巯基配位聚合物电化学催化的新型乙酰胆碱酯酶电化学传感器

Electrochemical Assay for Acetylcholinesterase Activity Detection Based on Unique Electro-catalytic Activity of Cu(II)-thiol Coordination Polymer

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