高效近红外聚集诱导发光纳米粒子用于生物成像的研究
收稿日期: 2016-08-24
网络出版日期: 2016-11-24
基金资助
项目受973计划（No.2013CB834701）、国家自然科学基金（Nos.51373063，51573068，21221063）、长江学者和创新研究团队（No.IRT101713018）资助.
Efficient Near-infrared AIE Nanoparticles for Cell Imaging
Received date: 2016-08-24
Online published: 2016-11-24
Supported by
Project supported by 973 Program (No. 2013CB834701), the Natural Science Foundation of China (Nos. 51373063, 51573068, 21221063) and Program for Chang Jiang Scholars and Innovative Research Team in University (No. IRT101713018).
纪光 , 闫路林 , 王慧 , 马莲 , 徐斌 , 田文晶 . 高效近红外聚集诱导发光纳米粒子用于生物成像的研究[J]. 化学学报, 2016 , 74(11) : 917 -922 . DOI: 10.6023/A16080430
Near-infrared fluorescence signals are highly desirable to acheieve high resolution in biological imaging. We encapsulated hydrophobic AIE (aggregation-induced emission) fluorophores into the biocompatible Pluronic F-127 NPs for cellular imaging and efficiently enhance the near-infrared AIE fluorophore emission. AIE molecule 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl) fumaronitrile (TPABDFN) with near-infrared emission was synthesized and selected as the fluorescence resonance energy transfer (FRET) acceptor. (2-p-tolylethene-1,1,2-triyl)tribenzene (TPE-Me) was a blue-emitting AIE molecule, which spectrum was matching with TPABDFN. TPE-Me@F127 NPs emission was 480 nm, TPABDFN@F127 NPs maximum absorption wavelength was also 480 nm, that the absorption had a large area of overlapping with the TPE-Me@F127 NPs emission spectrum and leaded to efficient energy transfer, so TPE-Me was selected as the FRET donor. By encapsulating both TPE-Me donor and TPABDFN acceptor simultaneously within the NPs, a significant FRET effect was induced. FRET pairs of different ratios was co-encapsulated into the F127 NPs to optimize the fluorescence signals. The maximum of fluorescence quantum yield was 19.9%, energy transfer efficiency was 43.5%. TPABDFN@F127 NPs only had weak fluorescence, but the TPABDFN/TPE-Me@F127 NPs showed bright fluorescence signal. Fluorescence resonance energy transfer contributed to the notable increase of acceptor emission The fluorescence quantum yield had 10-fold enhancement of the TPABDFN. In addition, the obtained TPABDFN/TPE-Me@F127 NPs showed a large Stokes shift of 265 nm, which can be used to avoid the interference between excitation and emission light, as well as the near-infrared emission spectrum away from the organism auto-fluorescence, which was beneficial for the bio-application. Fluorescent probe emission in the far red/near-infrared (FR/NIR) (650~900 nm) region for biological detection also can greatly reduce the damage to living body. And TPABDFN/TPE-Me@F127 NPs had low cytotoxicity, good biocompatibility, stability and anti-photobleaching. The TPABDFN/TPE-Me@F127 NPs achieved good imaging result on HepG2 cell cytoplasm.
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