高效近红外聚集诱导发光纳米粒子用于生物成像的研究

doi:10.6023/A16080430

化学学报 ›› 2016, Vol. 74 ›› Issue (11): 917-922.DOI: 10.6023/A16080430 上一篇下一篇

研究论文

高效近红外聚集诱导发光纳米粒子用于生物成像的研究

纪光, 闫路林, 王慧, 马莲, 徐斌, 田文晶

吉林大学化学学院超分子结构与材料国家重点实验室长春 130012

投稿日期:2016-08-24 发布日期:2016-11-24
通讯作者: 徐斌, 田文晶 E-mail:wjtian@jlu.edu.cn;xubin@jlu.edu.cn
基金资助:
项目受973计划（No.2013CB834701）、国家自然科学基金（Nos.51373063，51573068，21221063）、长江学者和创新研究团队（No.IRT101713018）资助.

Efficient Near-infrared AIE Nanoparticles for Cell Imaging

Ji Guang, Yan Lulin, Wang Hui, Ma Lian, Xu Bin, Tian Wenjing

State Key Laboratory of Supramolecular Structure and Materials, Jilin University, Changchun 130012

Received:2016-08-24 Published:2016-11-24
Supported by:
Project supported by 973 Program (No. 2013CB834701), the Natural Science Foundation of China (Nos. 51373063, 51573068, 21221063) and Program for Chang Jiang Scholars and Innovative Research Team in University (No. IRT101713018).

文章分享

1. 高效近红外聚集诱导发光纳米粒子用于生物成像的研究.PDF(1068KB)

摘要/Abstract

基于荧光共振能量转移机理（FRET），利用两亲性聚合物Pluronic F-127共包覆两种聚集诱导发光（Aggregation-induced Emission，AIE）材料TPABDFN和TPE-Me，制备了高效近红外发射TPABDFN/TPE-Me@F127纳米粒子.实验表明，这种聚合物纳米粒子具有很大的斯托克斯位移和较高的荧光量子效率，很好的单分散性、稳定性，以及较好的生物相容性和低的细胞毒性，对HepG2细胞进行荧光生物成像，得到很好的细胞成像效果.

关键词: 聚集诱导发光, 能量转移, 自组装, 纳米粒子, 生物成像

Near-infrared fluorescence signals are highly desirable to acheieve high resolution in biological imaging. We encapsulated hydrophobic AIE (aggregation-induced emission) fluorophores into the biocompatible Pluronic F-127 NPs for cellular imaging and efficiently enhance the near-infrared AIE fluorophore emission. AIE molecule 2-(4-bromophenyl)-3-(4-(4-(diphenylamino)styryl)phenyl) fumaronitrile (TPABDFN) with near-infrared emission was synthesized and selected as the fluorescence resonance energy transfer (FRET) acceptor. (2-p-tolylethene-1,1,2-triyl)tribenzene (TPE-Me) was a blue-emitting AIE molecule, which spectrum was matching with TPABDFN. TPE-Me@F127 NPs emission was 480 nm, TPABDFN@F127 NPs maximum absorption wavelength was also 480 nm, that the absorption had a large area of overlapping with the TPE-Me@F127 NPs emission spectrum and leaded to efficient energy transfer, so TPE-Me was selected as the FRET donor. By encapsulating both TPE-Me donor and TPABDFN acceptor simultaneously within the NPs, a significant FRET effect was induced. FRET pairs of different ratios was co-encapsulated into the F127 NPs to optimize the fluorescence signals. The maximum of fluorescence quantum yield was 19.9%, energy transfer efficiency was 43.5%. TPABDFN@F127 NPs only had weak fluorescence, but the TPABDFN/TPE-Me@F127 NPs showed bright fluorescence signal. Fluorescence resonance energy transfer contributed to the notable increase of acceptor emission The fluorescence quantum yield had 10-fold enhancement of the TPABDFN. In addition, the obtained TPABDFN/TPE-Me@F127 NPs showed a large Stokes shift of 265 nm, which can be used to avoid the interference between excitation and emission light, as well as the near-infrared emission spectrum away from the organism auto-fluorescence, which was beneficial for the bio-application. Fluorescent probe emission in the far red/near-infrared (FR/NIR) (650~900 nm) region for biological detection also can greatly reduce the damage to living body. And TPABDFN/TPE-Me@F127 NPs had low cytotoxicity, good biocompatibility, stability and anti-photobleaching. The TPABDFN/TPE-Me@F127 NPs achieved good imaging result on HepG2 cell cytoplasm.

Key words: aggregation-induced emission, energy transfer, self-assembly, nanoparticles, cell imaging

引用此文

纪光, 闫路林, 王慧, 马莲, 徐斌, 田文晶. 高效近红外聚集诱导发光纳米粒子用于生物成像的研究[J]. 化学学报, 2016, 74(11): 917-922.

Ji Guang, Yan Lulin, Wang Hui, Ma Lian, Xu Bin, Tian Wenjing. Efficient Near-infrared AIE Nanoparticles for Cell Imaging[J]. Acta Chim. Sinica, 2016, 74(11): 917-922.

导出引用 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

分享此文

参考文献

[1] Luo, S.; Zhang, E.; Su, Y. Biomaterials 2011, 32, 7127.
[2] Frangioni, J. V. Curr. Opin. Chem. Biol. 2003, 7, 626.
[3] Liu, J.; Geng, J.; Liu, B. Chem. Commun. 2013, 49, 1491.
[4] Ghoroghchian, P. P.; Frail, P. R.; Susumu, K. Proc. Natl. Acad. Sci. U. S. A. 2005, 102, 2922.
[5] Geng, J.; Li, K.; Pu, K. Y. Small 2012, 8, 2421.
[6] He, X.; Wang, K.; Cheng, Z. WIRES Nanomed Nano 2010, 2, 349.
[7] Lu, H. G.; Xu, B.; Tian, W. J. Angew. Chem. Int. Ed. 2016, 55, 155.
[8] Zhang, Y.; Wu, C. F.; Tian, W. J. RSC Adv. 2015, 5, 36837.
[9] Gao, G. B.; Gong, D. J.; Zhang, M. X. Acta Chim. Sinica 2016, 74, 363. (高冠斌, 龚德君, 张明曦, 孙涛垒, 化学学报, 2016, 74, 363.)
[10] Zrazhevskiy, P.; Sena, M.; Gao, X. Chem. Soc. Rev. 2010, 39, 4326.
[11] Gao, J.; Chen, K.; Luong, R. Nano Lett. 2011, 12, 281.
[12] Michalet, X.; Pinaud, F.; Bentolila, L. Science 2005, 307, 538.
[13] Cui, X. T.; Lv, Y. Y.; Liu, Y. Acta Chim. Sinica 2014, 72, 75. (崔晓腾, 吕玉洋, 刘颖, 吴伯岳, 化学学报, 2014, 72, 75.)
[14] Santra, S.; Zhang, P.; Wang, K. Anal. Chem. 2001, 73, 4988.
[15] Wu, X.; Chang, S.; Sun, X. Chem. Sci. 2013, 4, 1221.
[16] Shi, C.; Guo, Z.; Yan, Y. ACS Appl. Mater. Interfaces 2012, 5, 192.
[17] Gao, X.; Yang, L.; Petros, J. A. Curr. Opin. Chem. Biol. 2005, 16, 63.
[18] Resch-Genger, U.; Grabolle, M.; Cavaliere-Jaricot, S. Nat. Methods 2008, 5, 763.
[19] Smith, A.; Duan, H.; Mohs, A. Adv. Drug Deliver. Rev. 2008, 60, 1226.
[20] Jamieson, T.; Bakhshi, R.; Petrova, D. Biomaterials 2007, 28, 4717.
[21] Wang, L.; Tan, W. Nano Lett. 2006, 6, 84.
[22] Schadlich, A.; Caysa, H.; Mueller, T. ACS Nano 2011, 5, 8710.
[23] Lee, C. H. Cheng, S. H.; Wang, Y. J. Adv. Funct. Mater. 2009, 19, 215.
[24] Alt?no?lu, E.; Adair, J. H WIRES Nanomed. Nanobi. 2010, 2, 461.
[25] Yan, L. L.; Xu, B.; Tian, W. J. Nanoscale 2016, 8, 2471.
[26] Thomas, S.; Joly, G.; Swager, T. Chem. Rev. 2007, 107, 1339.
[27] Brasseur, N.; Nguyen, T.; Langlois, R. J. Med. Chem. 1994, 37, 415.
[28] Mei, J.; Leung, N.; Tang, B. Z. Chem. Rev. 2015, 115, 11718.
[29] Luo, J.; Xie, Z.; Lam, J. W. Chem. Commun. 2001, 18, 1740.
[30] Hong, Y.; Lam, J. W.; Tang, B. Z. Chem. Commun. 2009, 29, 4332.
[31] Wang, M.; Zhang, D.; Zhang, G. Chem. Commun. 2008, 37, 4469.
[32] Hong, Y.; Lam, J.; Tang, B. Z. Chem. Soc. Rev. 2011, 40. 5361.
[33] Wang, M.; Zhang, G.; Zhang, D. J. Mater. Chem. 2010, 20, 1858.
[34] Chen J. L.; Xu, B.; Tian, W. J. ACS Photonics 2015, 2, 313.
[35] Zhang, Y.; Xu, B.; Tian, W. J. Polym. Chem. 2014, 5, 3824.
[36] Qi, Q. K.; Xu, B.; Tian, W. J. Adv. Funct. Mater. 2015, 25, 4005.
[37] Zhang, J. B.; Xu, B.; Tian, W. J. Adv. Mater. 2014, 26, 739.
[38] Zhang, J. B.; Xu, B.; Tian, W. J. Chem. Commun. 2013, 49, 3878.
[39] Zhao, Z.; Geng, J.; Chang, Z. J. Mater. Chem. 2012, 22, 11018.
[40] Qin, W.; Ding, D.; Liu, J. Adv. Funct. Mater. 2012, 22, 771.
[41] Geng, J.; Li, K.; Ding, D. Small 2012, 8, 3655.
[42] Geng, J.; Li, K.; Qin, W. Small 2013, 9, 2012.
[43] Shi, H.; Liu, J.; Geng, J. J. Am. Chem. Soc. 2012, 134, 9569.
[44] Wang, M.; Gu, X.; Zhang, G. Anal. Chem. 2009, 81, 4444.
[45] Liu, L.; Zhang, G.; Xiang, J. Org. Lett. 2008, 10, 4581.
[46] Li, X.; Xu, B.; Tian, W. J. Anal. Chem. 2014, 86, 298.
[47] Ma, K.; Xu, B.; Tian, W. J. Anal. Bioanal. Chem. 2015, 407, 2625.
[48] Qian, J.; Zhu, Z. F.; Qin, A. J. Adv. Mater. 2015, 27, 2332.
[49] Wang, Y. L.; Hu, R. R.; Xu, W. Biomed. Opt. Express. 2015, 6, 3783.
[50] Jin, Y.; Ye, F.; Zeigler, M. ACS Nano 2011, 5, 1468.
[51] Chung, C. Y.-S.; Yam, V. W.-W. Chem. Sci. 2013, 4, 377.
[52] Xu, Y.; Zhang, H.; Li, F. J. Mater. Chem. 2012, 22, 1592.

高效近红外聚集诱导发光纳米粒子用于生物成像的研究

Efficient Near-infrared AIE Nanoparticles for Cell Imaging

PDF

补充材料

可视化

被引次数

摘要/Abstract

引用此文

分享此文

参考文献

相关文章 15

编辑推荐

相关统计

本文评价

[1]	魏文杰, 陈文龙, 戴晓彬, 燕立唐. 生物大分子介质中的反常扩散动力学理论^★[J]. 化学学报, 2023, 81(8): 967-978.
[2]	胡立伟, 刘宪虎, 刘春太, 宋延林, 李明珠. 光子晶体结构色材料的自组装制备与应用^★[J]. 化学学报, 2023, 81(7): 809-819.
[3]	郑奉斌, 王琨, 林田, 王英龙, 李国栋, 唐智勇. 金属有机骨架封装金属纳米粒子复合材料的制备及其催化应用研究进展^★[J]. 化学学报, 2023, 81(6): 669-680.
[4]	李兰英, 陶晴, 闻艳丽, 王乐乐, 郭瑞妍, 刘刚, 左小磊. 多聚腺嘌呤DNA探针及其生物传感应用^★[J]. 化学学报, 2023, 81(6): 681-690.
[5]	刘懿玮, 马良伟, 王巧纯, 马骧. 基于二芳基乙烯的光响应型室温磷光材料^★[J]. 化学学报, 2023, 81(5): 445-449.
[6]	苏东芮, 任小康, 于沄淏, 赵鲁阳, 王天宇, 闫学海. 酪氨酸衍生物调控酶催化路径可控合成功能黑色素^★[J]. 化学学报, 2023, 81(11): 1486-1492.
[7]	溥旭, 李泽娟, 石隽秋, 朱云卿, 杜建忠. 器官靶向的聚合物核酸载体研究进展^★[J]. 化学学报, 2023, 81(10): 1438-1446.
[8]	眭玉光, 周锦荣, 廖攀, 梁文杰, 徐海. 巨型给-受体分子开关化合物的合成及性质研究[J]. 化学学报, 2022, 80(8): 1061-1065.
[9]	刘斌, 陈磅宽. 基于联萘酚骨架的新型圆偏振发光材料的合成及性能探究[J]. 化学学报, 2022, 80(7): 929-935.
[10]	Jamshid Kadirkhanov, 钟峰, 张文建, 洪春雁. 聚合诱导自组装制备多腔室囊泡以及成核链段中亲溶剂片段的影响[J]. 化学学报, 2022, 80(7): 913-920.
[11]	赵晋源, 张乾, 王坚, 张琦, 李恒, 杜亚平. 活性氧捕获材料的研究进展[J]. 化学学报, 2022, 80(4): 570-580.
[12]	陈俊敏, 崔承前, 刘瀚林, 李国栋. 金属有机框架与Pt粒子复合材料催化喹啉选择性加氢性能研究^※[J]. 化学学报, 2022, 80(4): 467-475.
[13]	王志琴, 项博, 黄晓宇, 陆国林, 冯纯. 磷钨酸对对苯撑乙烯撑寡聚物-b-聚(2-乙烯基吡啶)自晶种行为的影响^※[J]. 化学学报, 2022, 80(3): 297-302.
[14]	张璐璐, 王媛媛, 朱贵楠, 戴文博, 赵紫璇, 赵盈, 支俊格, 董宇平. 含不同烷基链四苯基丁二烯衍生物的聚集诱导发光及力致变色性能[J]. 化学学报, 2022, 80(3): 282-290.
[15]	李嫣然, 王子贵, 汤朝晖. 水溶性IR-780聚合物用于线粒体靶向的光动力治疗^※[J]. 化学学报, 2022, 80(3): 291-296.