Study on Intercalation of DNA-Ethidium Complex into Layered Hydrotalcite

Acta Chimica Sinica ›› 2004, Vol. 62 ›› Issue (22): 2239-2243. Previous Articles Next Articles

DNA和菲啶鎓复合物嵌入水滑石的研究

李文卓¹, 陆军², 秦成刚¹, 陈接胜¹, 李连生¹, 黄佰渠²

1. 吉林大学无机合成与制备化学国家重点实验室, 长春, 130012;
2. 东北师范大学遗传和细胞研究所, 长春, 130024

投稿日期:2004-05-24 修回日期:2004-07-16 发布日期:2014-02-17
通讯作者: 陈接胜,E-mail:chemcj@mail.jlu.edu.cn;Fax:(+86)0431-5671974 E-mail:chemcj@mail.jlu.edu.cn

Study on Intercalation of DNA-Ethidium Complex into Layered Hydrotalcite

LI Wen-Zhuo¹, LU Jun², QIN Cheng-Gang¹, CHEN Jie-Sheng¹, LI Lian-Sheng¹, HUANG Bai-Qu²

1. State Key Laboratory of Inorganic Synthesis and Preparative Chemistry, College of Chemistry, Jilin University, Changchun 130012;
2. Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024

Received:2004-05-24 Revised:2004-07-16 Published:2014-02-17

Abstract

A new composite compound HT-DNA-ET has been prepared through intercalation of ethidium and DNA molecule into the layers of hydrotalcite (HT). The HT-DNA-ET compound has been investigated by various analytical methods such as X-ray diffraction, IR, UV-vis and circular dichroism spectroscopy, element and thermogravimetric analyses. The interlayer distance of the HT-DNA-ET compound is 4.6 nm, larger than that of the pristine HT. Taking into account the structure of the DNA molecule and the ET molecule, it was proposed that there are two layers of DNA-ET composite between two adjacent HT sheets. The DNA molecules in the HT-DNA-ET compound retain their chemical and biological integrity. The HT-DNA-ET compound gave rise to photoluminescence of λ_em,max=610 nm under the excitation of a laser light of λ_ex=488 nm.

Key words: DNA, ethidium bromide(HT), layed hydroxide, intercalation, photoluminescence

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LI Wen-Zhuo, LU Jun, QIN Cheng-Gang, CHEN Jie-Sheng, LI Lian-Sheng, HUANG Bai-Qu. Study on Intercalation of DNA-Ethidium Complex into Layered Hydrotalcite[J]. Acta Chimica Sinica, 2004, 62(22): 2239-2243.

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DNA和菲啶鎓复合物嵌入水滑石的研究

Study on Intercalation of DNA-Ethidium Complex into Layered Hydrotalcite

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